Human CD3 GMP Antibody GMP

Key Product Details

Product Specifications

Applications for Human CD3 GMP Antibody

Formulation, Preparation, and Storage

Background: CD3

The Bio-Techne CD3 GMP antibody is derived from the OKT3 clone and binds specifically to the epsilon subunit of the CD3 complex. CD3 (cluster of differentiation 3) is a protein complex and T cell co-receptor that is critical for the activation of T cells. Specifically, the CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulin super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum.

Additional CD3 Products

• All Products for CD3
• CD3 Cell Selection and Detection Kits
• CD3 ELISA Kits
• CD3 Primary Antibodies

Manufacturing Specifications

GMP Antibodies
R&D Systems, a Bio-Techne Brand's GMP Antibodies are produced according to relevant sections of the following documents: USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products, and Eu. Ph. 5.2.12, Raw Materials of Biological Origin for the Production of Cell-based and Gene Therapy Medicinal Products.
R&D Systems' quality focus includes:

• Manufactured and tested under an ISO 9001:2015 and ISO 13485:2016 certified quality system
• Documented processes and QA control of documentation and process changes
• Personnel training programs
• Raw material inspection and supplier qualification/monitoring
• Fully validated equipment, processes and test methods
• Equipment calibration monitored and scheduled using a computerized calibration program
• Facility maintenance, safety programs and pest control
• Variances dispositioned using material review process
• Robust product stability program

R&D Systems strives to provide our customers with the analytical characteristics of each product so that customers may determine whether our products are appropriate for their research. The Certificate of Analysis provided contains the following lot specific information:

• Purity (SDS-PAGE)
• Aggregation (SEC)
• Endotoxin (LAL method, tested per USP<85> and Ph. Eur. 2.6.14 guidelines)
• Residual Host Cell Protein and Host Cell DNA
• Mycoplasma testing
• Post-bottling lot-specific bioassay results (compliance with an established range) and results of microbial testing according to USP <71>

Additional testing and documentation requested by the customer can be arranged at an additional cost.
Production records and facilities are available for examination by appropriate personnel on-site at R&D Systems in Minneapolis, Minnesota USA.
R&D Systems sells GMP grade products for preclinical or clinical ex vivo use. They are not for in vivo use. Please read the following End User Terms prior to using this product.