Human CaM Kinase I Alexa Fluor® 750-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7899S
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Alexa Fluor 750 (Excitation = 749 nm, Emission = 775 nm)
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human CaM Kinase Iα
Ser176-Arg254 (Ala224Ser)
Accession # Q14012
Ser176-Arg254 (Ala224Ser)
Accession # Q14012
Specificity
Detects recombinant human CaM Kinase I alpha in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human CaMKIV is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications
Application
Recommended Usage
Immunohistochemistry
Optimal dilution of this antibody should be experimentally determined.
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: CaM Kinase I
CaMKI (Calcium/Calmodulin-dependent kinase I) is a 37-43 kDa monomeric and cytosolic member of the CaMK subfamily, Ser/Thr protein kinase family, protein kinase superfamily of molecules. It is found in multiple cell types, including adrenal cortical cells, neurons and macrophages/osteoclast precursors. CaMKI is associated with the cellular Ca++ signaling pathway. Upon entry into the cell, calcium levels are sensed by calmodulin. Upon Ca++ binding, calmodulin (and CaMK kinase) interacts with a Ser/Thr protein kinase termed CaMKIa. CaMKIa is of particular interest in neurons and cells of the zona glomerulosa. In neurons, CaMKIa activation results in the recruitment of AMPA-Rs and the promotion of axon growth (vs.dendrite outgrowth which is promoted by CaMKIg). In the adrenal gland, CaMKIa regulates CYP11B2 transcription, and thus the ability to synthesize aldosterone. Human CaMKI, the isoform used for immunization, is 370 amino acids (aa) in length (SwissProt #:Q14012). It contains one protein kinase domain (aa 20-276) followed by a calmodulin-binding region (aa 296-317) and an NES (aa 315-321). Over aa 178-257, human and mouse CaMKI are identical in amino acid sequence. There are three additional CaMKI isoforms (b, g, d), all the product of distinct genes. Nevertheless, over the same aa sequence, these three isoforms (b, g, d) share 80%, 81% and 97% aa sequence identity with CaMKI(a), respectively.
Long Name
Calcium/Calmodulin-dependent Protein Kinase I
Alternate Names
calcium/calmodulin-dependent protein kinase I, calcium/calmodulin-dependent protein kinase type 1, CaM kinase I, CaM kinase I alpha, CaMKI, CaM-KI, caMKI-alpha, EC 2.7.11, EC 2.7.11.17, MGC120317, MGC120318
UniProt
Additional CaM Kinase I Products
Product Specific Notices
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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