Human C-Peptide Antibody

Name: Human C-Peptide Antibody
Brand: R&D Systems
SKU: MAB11798

R&D Systems, part of Bio-Techne | Catalog # MAB11798

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Product Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Key Product Details

Species Reactivity:	Human
Immunogen:	Synthetic peptide corresponding to human C-Peptide (proteolytic fragment derived from Insulin 57-87) Accession # P01308
Applications:	Immunohistochemistry
Label:	Unconjugated
Source:	Recombinant Monoclonal Rabbit IgG Clone # 3347a
Specificity:	Detects a synthetic peptide for Human Insulin C-peptide in Direct ELISA.

View Full Technical Details for Human C-Peptide Antibody

Technical Data
Citations / Reviews

Applications for Human C-Peptide Antibody

	Recommended Concentration	Sample
Immunohistochemistry	0.25-10 µg/mL	Immersion fixed paraffin-embedded sections of human pancreas

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human C-Peptide Antibody

	Immunohistochemistry-Paraffin Detection of Insulin in Human Pancreas. C-Peptide was detected in immersion fixed paraffin-embedded sections of human pancreas using Rabbit Anti-Human Insulin Monoclonal Antibody (Catalog # MAB11798) at 0.25 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (VCTS021). Tissue was stained using the HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of pancreatic islets. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Reconstitution	Reconstitute lyophilized material at 0.2mg/ml in sterile PBS. For liquid material, refer to CoA for concentration. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping	Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: Insulin

C-peptide is a 31-amino-acid peptide that is cleaved from proinsulin during insulin biosynthesis, playing a crucial role as a byproduct of insulin processing. Though initially considered biologically inert, recent evidence highlights its significant physiological effects, including promoting microvascular blood flow and acting on cellular pathways linked to inflammation and oxidative stress. Unlike insulin, C-peptide is not rapidly metabolized by the liver, making its steady degradation in the kidneys a reliable marker for endogenous insulin production. This feature is particularly valuable in the clinical evaluation of beta-cell function and insulin secretion in both type 1 and type 2 diabetes. Additionally, altered levels of C-peptide have been associated with various disease states, including metabolic syndrome, cardiovascular complications, and diabetic nephropathy, suggesting potential roles as both a biomarker and therapeutic target. The physiological and pathophysiological significance of C-peptide continues to be an active area of research, underpinning its importance in metabolic and vascular health.

Item	Value
References	Wahren J, Ekberg K, Johansson J, Henriksson M, Pramanik A, Johansson BL, Rigler R, Jörnvall H. Role of C-peptide in human physiology. Am J Physiol Endocrinol Metab. 2000 May;278(5):E759-68. doi: 10.1152/ajpendo.2000.278.5.E759. PMID: 10780930. Chen J, Huang Y, Liu C, Chi J, Wang Y, Xu L. The role of C-peptide in diabetes and its complications: an updated review. Front Endocrinol (Lausanne). 2023 Sep 7;14:1256093. doi: 10.3389/fendo.2023.1256093. PMID: 37745697; PMCID: PMC10512826. Yosten GL, Maric-Bilkan C, Luppi P, Wahren J. Physiological effects and therapeutic potential of proinsulin C-peptide. Am J Physiol Endocrinol Metab. 2014 Dec 1;307(11):E955-68. doi: 10.1152/ajpendo.00130.2014.
Entrez Gene IDs	3630 (Human); 16333 (Mouse); 397415 (Porcine); 280829 (Bovine); 483665 (Canine)
Alternate Names	IDDM2, ILPR, INS, IRDN, Insulin, MODY10, proinsulin

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Human C-Peptide Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB11798

Key Product Details

Applications for Human C-Peptide Antibody

Scientific Data Examples for Human C-Peptide Antibody

Immunohistochemistry-Paraffin

Formulation, Preparation and Storage

Reconstitution Calculator

Background: Insulin

Customer Reviews for Human C-Peptide Antibody

Product Documents for Human C-Peptide Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

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