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Human BMP‑3b/GDF‑10 Antibody

Catalog # AF1543 | R&D Systems, Inc. a Bio-Techne Brand
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AF1543
AF1543-SP

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Summary for Human BMP‑3b/GDF‑10 Antibody

Immunogen

E. coli-derived recombinant human BMP‑3b/GDF‑10
Gln369-Arg478
Accession # Q5VSQ8

Specificity

Detects human BMP‑3b/GDF‑10 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human (rh) BMP-3 is observed and less than 2% cross-reactivity with rhBMP-2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human BMP‑3b/GDF‑10 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human prostate cancer tissue

Western Blot

0.1 µg/mL
Sample: Recombinant Human BMP‑3b/GDF‑10 (Catalog # 1543-BP)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 1 publication using AF1543 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMP-3b/GDF-10

BMP-3b, also known as GDF-10, belongs to the transforming growth factor beta (TGF-beta) superfamily which includes the TGF-beta s, bone morphogenetic proteins (BMPs), growth differentiation factors (GDFs), Activins, Inhibins, Leftys, Nodal, Mullerian inhibitory substance (MIS) and the glial cell line-derived neurotrophic factors (GDNFs) (1). TGF-beta family members are synthesized and secreted as homodimeric or heterodimeric prepropeptides that are cleaved by proprotein convertases such as furin to generate the carboxy-terminal mature dimeric protein that contains the characteristic conserved cysteine residues involved in the formation of the cysteine knot domain. Within pro-BMP-3b, three dibasic cleavage sites have been identified. Mature BMP-3B is a disulfide-linked homodimer of the C-terminal 110 amino acids. Among TGF-beta  family members, BMP-3b is most closely related to BMP-3, sharing 83% and 30% amino acid sequence identity in their mature and pro regions, respectively (2, 3). BMP-3b is highly conserved across animal species, the aa sequence of mature human BMP-3b is 98% identical with that of the mouse or rat proteins (2, 3). BMP-3b is expressed in developing skeletal structures of the craniofacial region and the vertebral column, as well as in the adult trachea, aorta, and most abundantly in the cerebellum and uterus (2‑4). Interestingly, in the knock-out mice, no obvious abnormalities have been found in these tissues (4). The biological function of BMP-3b is yet unknown, however studies have implicated it in the differentiation of osteoblasts, augmenting BMP-2 activity (5, 6).

References

  1. Miyazono, K. et al. (2001) J. Cell Physiol. 187:265.
  2. Cunningham, N.S. et al. (1995) Growth Factors 12:99.
  3. Takao, M. et al. (1996) Biochem. Biophys. Res. Comm. 219:656.
  4. Zhao, R. et al. (1999) Dev. Biol. 212:68.
  5. Hino, J. et al. (1999) Biochem .Biophys. Res. Comm. 256:419.
  6. Kaihara, S. et al. (2003) Life Sci. 72:1683.

Long Name

Bone Morphogenetic Protein 3b

Alternate Names

BMP3b, GDF-10, GDF10

Entrez Gene IDs

2662 (Human); 14560 (Mouse)

Gene Symbol

GDF10

UniProt

Product Documents for Human BMP‑3b/GDF‑10 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human BMP‑3b/GDF‑10 Antibody

For research use only

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Reconstitution Calculator

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