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Human APP695+1 Antibody

Catalog # AF890 | R&D Systems, Inc. a Bio-Techne Brand
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AF890
AF890-SP

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Summary for Human APP695+1 Antibody

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human APP695+1 frameshift mutant
Met1-Arg336
Accession # NP_958817

Specificity

Detects human APP695+1 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human APP695+1 Antibody

APP695+1in Human Brain by Immunohistochemistry (IHC-P).

APP695+1in Human Brain.

APP695+1was detected in immersion fixed paraffin-embedded sections of human Alzheimer's brain using Goat Anti-Human APP695+1Antigen Affinity-purified Polyclonal Antibody (Catalog # AF890) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human APP695+1 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human Alzheimer's brain

Western Blot

0.1 µg/mL
Sample: Recombinant Human APP695+1
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 3 using AF890 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: APP695+1

Amyloid precursor protein (APP) is a type I membrane protein with several human isoforms due to alternative splicing. APP770, 751, and 733 contain a Kunitz protease inhibitor (KPI) domain (residue 291‑342) and APP695 does not. APP is a cell surface molecule with many functions. It can be processed proteolytically in two different pathways. In one pathway, beta- and gamma-secretase cleave at the  beta site between residue 670 and 671 and the gamma site between residue 711 and 714 to produce beta‑amyloid peptide (A beta40 and A beta42), a major component in plaques found in brains of patients with Alzheimer's disease (1). The other pathway involves alpha-secretase that cleaves residues between 687 and 688. It is antiamyloidogenic due to its benign character and the prevention of the A beta peptide formation (2). Soluble APP containing the KPI domain, also referred to as protease nexin II, is a potent inhibitor of serine proteases and may have additional functions. For example, it may regulate the contact face of blood coagulation and limit thrombosis specially in brain due to its localization and coagulation factor XI inhibiting activity (3, 4).

References

  1. Haass, C. (2004) EMBO J. 23:483.
  2. Lichtenthaler, S. F. and C. Haass (2004) J. Clin. Invest. 113:1384.
  3. Badellino, K.O. and P.N. Walsh (2000) Biochemistry 39:4769.
  4. Xu, F. et al. (2005) Proc. Natl. Acad. Sci USA. 102:18135.

Long Name

Amyloid Precursor Protein 695+1 Frameshift Mutant

Entrez Gene IDs

351 (Human); 11820 (Mouse)

Gene Symbol

APP

UniProt

Additional APP695+1 Products

Product Documents for Human APP695+1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human APP695+1 Antibody

For research use only

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