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Human APP695+1 Antibody

Bio-Techne includes R&D Systems | Catalog # MAB890

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MAB890
MAB890-SP

Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 128715

Product Summary for Human APP695+1 Antibody

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human APP695+1 frameshift mutant
Leu18-Arg336 (predicted)
Accession # NP_958817

Specificity

Detects human APP695+1 in Western blots. It recognizes a common epitope present on APP695+1 and APP770 found within the segments L18-R288 or P365-R411 of APP770 (Accession # P05067).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human APP695+1 Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human APP/Protease Nexin II (Catalog # 3466-PI), see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Recombinant Human APP695+1
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: APP695+1

Amyloid precursor protein (APP) is a type I membrane protein with several human isoforms due to alternative splicing. APP770, 751, and 733 contain a Kunitz protease inhibitor (KPI) domain (residue 291‑342) and APP695 does not. APP is a cell surface molecule with many functions. It can be processed proteolytically in two different pathways. In one pathway, beta- and gamma-secretase cleave at the  beta site between residue 670 and 671 and the gamma site between residue 711 and 714 to produce beta‑amyloid peptide (A beta40 and A beta42), a major component in plaques found in brains of patients with Alzheimer's disease (1). The other pathway involves alpha-secretase that cleaves residues between 687 and 688. It is antiamyloidogenic due to its benign character and the prevention of the A beta peptide formation (2). Soluble APP containing the KPI domain, also referred to as protease nexin II, is a potent inhibitor of serine proteases and may have additional functions. For example, it may regulate the contact face of blood coagulation and limit thrombosis specially in brain due to its localization and coagulation factor XI inhibiting activity (3, 4).

References

  1. Haass, C. (2004) EMBO J. 23:483.
  2. Lichtenthaler, S. F. and C. Haass (2004) J. Clin. Invest. 113:1384.
  3. Badellino, K.O. and P.N. Walsh (2000) Biochemistry 39:4769.
  4. Xu, F. et al. (2005) Proc. Natl. Acad. Sci USA. 102:18135.

Long Name

Amyloid Precursor Protein 695+1 Frameshift Mutant

Entrez Gene IDs

351 (Human); 11820 (Mouse)

Gene Symbol

APP

UniProt

Additional APP695+1 Products

Product Documents for Human APP695+1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human APP695+1 Antibody

For research use only

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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