Human AP-2 gamma Antibody

Detection of Human AP-2 gamma by Western Blot.

Western Blot shows lysates of MCF-7 human breast cancer cell line, T47D human breast cancer cell line, MDA-MB-468 human breast cancer cell line and SK-BR-3 human breast cancer cell line. PVDF membrane was probed with 2 µg/ml of Goat Anti-Human AP-2 gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5059) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for AP-2 gamma at approximately 49 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

AP-2 gamma in Human Breast Cancer Tissue.

AP-2 gamma was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 10 µg/mL Goat Anti-Human AP-2 gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5059) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of AP-2 gamma by Immunocytochemistry/ Immunofluorescence

Characterization of Rhesus PSCs after Reprogramming to Naive Pluripotency(A) Phase contrast and immunostaining of naive pluripotency markers, TFAP2C, KLF4, and TFE3 in LyonES-tGFP-(S3) cells, before (primed rhesus PSCs) and after reprogramming to the naive state (E-NHSM, NHSM-v, 4i/L/b, TL2i, t2iLGöY, LCDM (EPS), and TL-CDK8/19i) (confocal imaging). Scale bars, 50 μm.(B) Immunostaining of 5′-methylcytosine (5mC), 5′-hydroxymethylcytosine (5hmC), H3K4me3, H3K9me3, H2AK119Ub, and H3K27me3 in LyonES-tGFP-(S3) cells, before (primed) and after reprogramming to the naive state (4i/L/b, TL2i, and t2iLGöY) (confocal imaging). Scale bars, 50 μm.(C) Activity of the proximal and distal enhancers (PE and DE, respectively) of OCT4 measured in a luciferase assay after transient transfection in primed PSCs, E-NHSM, NHSM-v, 4i/L/b, TL2i, t2iLGöY, and LCDM cells.(D) Principal-component analysis (PCA) for primed PSCs, E-NHSM, NHSM-v, 4i/L/b, TL2i, t2iLGöY, LCDM, and TL-CDK8/19i populations based on RNA sequencing (RNA-seq) data.(E) PCA for primed PSCs, E-NHSM, NHSM-v, 4i/L/b, TL2i, t2iLGöY, LCDM, and TL-CDK8/19i populations based on bulk RNA-seq and single-cell RNA-seq data of cynomolgus epiblast from E6 to E9 embryos (Nakamura et al., 2016). Image collected and cropped by CiteAb from the following open publication (https://linkinghub.elsevier.com/retrieve/pii/S2213671120304999), licensed under a CC-BY license. Not internally tested by R&D Systems.