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Key Product Details

Species Reactivity

Non-species specific

Applications

Immunohistochemistry, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 2H11cc

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with purified peroxidase from horseradish.

Specificity

Horseradish peroxidase isoforms with pI from 3.5 to 9.5. NB110-8035 does not influence enzymatic activity upon immune complex formation.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Description

Novus Biologicals Mouse HRP Antibody (2H11cc) - BSA Free (NB110-8035) is a monoclonal antibody validated for use in IHC and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.

Applications for HRP Antibody (2H11cc) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

1:10-1:500

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: HRP

Horseradish Peroxidase (HRP) is an enzyme commonly used as an indicator for chemical reactions which produce peroxide. The enzyme is routinely conjugated to antibodies for use in enzyme-based immunoassay systems. HRP functions in the removal of H2O2 (hydrogen peroxide), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue. HRP binds 1 heme B (iron-protoporphyrin IX) group and 2 calcium ions per subunit. Subunits are monomeric.

Alternate Names

EC 1.11.1.7, Horseradish Peroxidase, HPRC 1, HPRC1, HRPA2, HRPC 2, HRPC2, HRPE5, HRPN, Neutral peroxidase, Peroxidase A2, Peroxidase C1A, Peroxidase C1B, Peroxidase C2, Peroxidase C3, Peroxidase E5, Peroxidase N, PRXC1A, PRXC1B, PRXC2, PRXC3

Additional HRP Products

Product Documents for HRP Antibody (2H11cc) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HRP Antibody (2H11cc) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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