HPV16/HPV18 E6 Antibody (HPV16/1295 + HPV18/1297)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07675

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Virus

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # HPV16/1295 + HPV18/1297

Concentration

0.2 mg/ml

View all HPV16/HPV18 E6 Antibodies »

Product Specifications

Immunogen

HPV18 E6-beta-galactosidase fusion protein

Reactivity Notes

Type 16 of Human Papilloma Virus (HPV-16)

Localization

Nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08500)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for HPV16/HPV18 E6 Antibody (HPV16/1295 + HPV18/1297)

HPV16/HPV18 E6 Antibody (HPV16/1295 + HPV18/1297) HPV16/HPV18 E6 Antibody (HPV16/1295 + HPV18/1297) - Azide and BSA Free

HPV16/HPV18 E6 Antibody (HPV16/1295 + HPV18/1297) - Azide and BSA Free

Formalin-fixed, paraffin-embedded human HNSCC stained with HPV16E6 & HPV18E6 Mouse Monoclonal Antibody (HPV/16/18).

Applications for HPV16/HPV18 E6 Antibody (HPV16/1295 + HPV18/1297)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.5 - 1 ug/ml

Immunohistochemistry-Paraffin

1 - 2 ug/ml
Application Notes
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: HPV16/HPV18 E6

Human Papilloma virus (HPV) is responsible for the development of type and tissue specific papilloma in the oral cavity and in the larynx. In the skin papilloma virus can cause different types of warts and in the ano-genital region it is associated with condyloma or carcinoma. HPV types 1, 6 and 11 usually are associated with benign transformations of the tissues, while e.g. HPV types 16, 18 and 31 seem to be responsible for the development of some carcinoma like cervical carcinoma. The role of HPV in non-melanoic tumours of the skin is under discussion. Protein E6 is a Transcriptional transactivator, binding double stranded DNA. It has transforming activity inactivating, with E6-AP ubiquitin-protein ligase, the human TP53/p53 tumor suppressor protein by targeting it for degradation. Clinical relevance: Detection of HPV in cervical smears and biopsies. Uses: Analysis of E6 expression in cell transformation studies.

Alternate Names

E6, Human Papilloma Virus, Human papillomavirus type 16 E6, Human papillomavirus type 18 E6, Protein E6

Additional HPV16/HPV18 E6 Products

Product Documents for HPV16/HPV18 E6 Antibody (HPV16/1295 + HPV18/1297)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HPV16/HPV18 E6 Antibody (HPV16/1295 + HPV18/1297)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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