HOXB4 Antibody (JE36-65)

Western Blot: HOXB4 Antibody (JE36-65) [NBP3-32440]

Western Blot: HOXB4 Antibody (JE36-65) [NBP3-32440] - Western blot analysis of HOXB4 on different lysates with Rabbit anti-HOXB4 antibody (NBP3-32440) at 1/1,000 dilution.

Lane 1: Jurkat cell lysate
Lane 2: HL-60 cell lysate
Lane 3: PC-12 cell lysate
Lane 4: Mouse liver tissue lysate

Lysates/proteins at 30 ug/Lane.

Predicted band size: 28 kDa
Observed band size: 34 kDa

Exposure time: 5 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32440) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: HOXB4 Antibody (JE36-65) [NBP3-32440]

Immunohistochemistry: HOXB4 Antibody (JE36-65) [NBP3-32440] - Immunohistochemical analysis of paraffin-embedded mouse embryo cartilage tissue with Rabbit anti-HOXB4 antibody (NBP3-32440) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32440) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow Cytometry: HOXB4 Antibody (JE36-65) [NBP3-32440]

Flow Cytometry: HOXB4 Antibody (JE36-65) [NBP3-32440] - Flow cytometric analysis of HL-60 cells labeling HOXB4.

Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32440, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).