hnRNP C1 + C2 Antibody (9G1) - BSA Free

Immunohistochemistry: hnRNP C1 + C2 Antibody (9G1) [NBP3-26221]

Immunohistochemistry: hnRNP C1 + C2 Antibody (9G1) [NBP3-26221] - NBP3-26221 diluted at 1:300 and staining in paraffin-embedded human braintissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.Secondary antibody only control: uses 1% BSA instead of primary antibody

Flow Cytometry: hnRNP C1 + C2 Antibody (9G1) [NBP3-26221]

Flow Cytometry: hnRNP C1 + C2 Antibody (9G1) [NBP3-26221] - Overlay Peak curve showing MCF7 cells stained with NBP3-26221 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was Rabit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Immunocytochemistry/Immunofluorescence: hnRNP C1 + C2 Antibody (9G1) [NBP3-26221]

Immunocytochemistry/Immunofluorescence: hnRNP C1 + C2 Antibody (9G1) [NBP3-26221] - Staining of Hela cell with NBP3-26221 at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).