HM74A/PUMA-G/GPR109A/NIACR1 Antibody - BSA Free

Immunohistochemistry-Paraffin: HM74A/PUMA-G/GPR109A/NIACR1 Antibody [NBP1-92180]

Immunohistochemistry-Paraffin: HM74A/PUMA-G/GPR109A/NIACR1 Antibody [NBP1-92180] - Staining of human tonsil shows strong cytoplasmic positivity in non-germinal center cells.

Western Blot: HM74A/PUMA-G/GPR109A/NIACR1 Antibody - BSA Free [NBP1-92180] -

Niacin can activate the GPR109A/NRF2/autophagy signal pathway. The cells were collected at 0, 3, 6, 12, and 24 h to extract the total protein. The total protein was prepared and subjected to Western blotting using GPR109A, NRF-2, HO-1, and beta-tubulin antibodies. T-NRF-2 means total NRF-2. (a–d) The protein levels of GPR109A, NRF-2, and HO-1. The cells from different experimental groups were treated with niacin or shRNA+niacin for 24 h, and then, the total protein was collected. N-NRF-2 means NRF-2 in the nucleus. C-NRF-2 means NRF-2 in the cytoplasm. (e–h) The protein levels of GPR109A, C-NRF-2, N-NRF-2, and HO-1. Each immunoreactive band was digitized and expressed as a ratio of the beta-tubulin level. (i) The immunofluorescence results of the assay for NRF-2. The scale length in the figure is 200 μM. (j) The relative fluorescence intensity of NRF-2/ARE. The mRNA levels were determined by qRT-PCR. (k) The mRNA levels of ATG12, ATG4D, p62, ATG5, ULK1, ATG4B, Beclin, LC3B, and ATG7 were normalized to the level of beta-actin. The values are presented as the means +/- SD (∗ p < 0.05 and ∗∗ p < 0.01). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32397071), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: HM74A/PUMA-G/GPR109A/NIACR1 Antibody - BSA Free [NBP1-92180] -

(A) Effects of high glucose concentration (25.2 mM) on the mRNA expression of GPR109a in Caco-2 cells vs. low glucose concentration (5.6 mM). Results are expressed as means +/- standard error of the mean (SEM), n = 3, control = 5.6 mM glucose, control vs. 25.2 mM glucose, * p < 0.05; (B) Effects of high glucose (25.2 mM) on GPR109a protein expression in Caco-2 cells vs. control 5.6 mM glucose concentrations. Results are expressed as means +/- SEM, n = 6, control = 5.6 mM vs. 25.2 mM, * p < 0.05. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26371038), licensed under a CC-BY license. Not internally tested by Novus Biologicals.