HLA A2 Antibody (BB7.2) - Chimeric - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-43553
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # BB7.2
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
The original antibody was generated by immunizing BALB/c mice with papain solubilized HLA A and B7 antigens. (Uniprot# P04439)
Specificity
This antibody binds the carboxy terminus of the alpha2-helix of class I MHC molecule and a turn on one of the underlying beta-strands.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Scientific Data Images for HLA A2 Antibody (BB7.2) - Chimeric - BSA Free
Western Blot: HLA A2 Antibody (BB7.2) - Chimeric - BSA Free [NBP3-43553] -
Western Blot: HLA A2 Antibody (BB7.2) - Chimeric - BSA Free [NBP3-43553] - Daudi cells lysate (35ug protein in RIPA buffer) was resolved via SDS-PAGE, and the subsequent blot was probed with NBP3-43553 at 0.3ug/ml before detection using an appropriate secondary antibody. A primary incubation of 1 hour was used, and proteins were detected by chemiluminescence.
Immunocytochemistry /Immunofluorescence: HLA A2 Antibody (BB7.2) - Chimeric - BSA Free [NBP3-43553] -
Immunocytochemistry /Immunofluorescence: HLA A2 Antibody (BB7.2) - Chimeric - BSA Free [NBP3-43553] - Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix coverslips stained with NBP3-43553 (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, NBP3-43553, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.Applications for HLA A2 Antibody (BB7.2) - Chimeric - BSA Free
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: HLA A2
Alternate Names
A-10 alpha chain, FLJ26655, HLA class I histocompatibility antigen, A-1 alpha chain, HLA class I histocompatibility antigen, A-28 alpha chain, HLA class I histocompatibility antigen, A-9 alpha chain, HLAA, major histocompatibility complex, class I, A, MHC class I antigen A*1, MHC class I antigen A*11, MHC class I antigen A*80
Additional HLA A2 Products
Product Documents for HLA A2 Antibody (BB7.2) - Chimeric - BSA Free
Product Specific Notices for HLA A2 Antibody (BB7.2) - Chimeric - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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