HKDC1 Antibody (HL3464)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-48816
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL3464
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fragment of human HKDC1.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit HKDC1 Antibody (HL3464) (NBP3-48816) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for HKDC1 Antibody (HL3464)
Immunohistochemistry-Paraffin: HKDC1 Antibody (HL3464) [NBP3-48816] -
HKDC1 antibody [HL3464] detects HKDC1 protein by immunohistochemical analysis.Sample: Paraffin-embedded human tissues.HKDC1 stained by HKDC1 antibody [HL3464] (NBP3-48816) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 minCorresponding RNA levels (RPKM) in the tissues are based on NCBI database.
Western Blot: HKDC1 Antibody (HL3464) [NBP3-48816] -
Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HKDC1 antibody [HL3464] (NBP3-48816) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Immunocytochemistry/ Immunofluorescence: HKDC1 Antibody (HL3464) [NBP3-48816] -
HKDC1 antibody [HL3464] detects HKDC1 protein at mitochondria by immunofluorescent analysis.Sample: AsPC-1 cells were fixed in ice-cold MeOH for 5 min.Green: HKDC1 stained by HKDC1 antibody [HL3464] (NBP3-48816) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .Applications for HKDC1 Antibody (HL3464)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: HKDC1
Alternate Names
EC 2.7.1, EC 2.7.1.1, FLJ22761, FLJ37767, hexokinase domain containing 1, Hexokinase domain-containing protein 1, MGC125688, putative hexokinase HKDC1
Gene Symbol
HKDC1
Additional HKDC1 Products
Product Documents for HKDC1 Antibody (HL3464)
Product Specific Notices for HKDC1 Antibody (HL3464)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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