Histone Nucleosome Core Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-745

Novus Biologicals, part of Bio-Techne
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NB100-745
NB100-745-0.025mg
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Key Product Details

Species Reactivity

Bovine

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Histone Nucleosome Core Antibodies »

Product Specifications

Immunogen

Intact calf H-3 complexed to rRNA.

Reactivity Notes

Although not tested this antibody may react with Human and other species.

Specificity

This is specific for Histone-Core. The antibody binds to H1, H2B, H3 and H4. There is no evidence to suggest that the antibody can bind to H2A.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Histone Nucleosome Core Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
Immunoprecipitaion, Western Blot. Although not tested this antibody may be useful in Immunoflourescence.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Histone Nucleosome Core

In diploid eukaryotic cells, the chromatin fibers are about 20nM in diameter. They consist of two major components in equal amounts, DNA and basic proteins called histones. Histones are believed to be regularly arranged in the deep grove of the DNA helix. The recurring positive charges of the histones form electrostatic associations with the negatively charged phosphate groups of DNA making the DNA more stable and flexible. This allows for the supercoiling of the chromatin fibers. The histone core protein is an octamer consisting of 2 subunits of each of histone 2A, 2B, 3 and 4. In conjunction with the linker histone H1 and the nonhistone chromosomal proteins, it is responsible for the binding and compacting of DNA into chromatin. The sequences of the core histones are highly conserved from species to species.

Additional Histone Nucleosome Core Products

Product Documents for Histone Nucleosome Core Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Histone Nucleosome Core Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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