Histone H3 [Trimethyl Lys36] Antibody (RM155)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61530
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Human, Vertebrate
Applications
Chromatin Immunoprecipitation (ChIP), ELISA, Multiplex Immunoassay, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # RM155
Concentration
1 mg/ml
Product Specifications
Immunogen
This Histone H3 [Trimethyl Lys36] antibody (RM155) was raised against a trimethyl-peptide corresponding to Histone H3 [Trimethyl Lys36]
Modification
Trimethyl Lys36
Specificity
This Histone H3 [Trimethyl Lys36] antibody (RM155) reacts to Histone H3 trimethylated at Lysine 36. No cross reactivity with monomethylated Lysine 36 or dimethylated Lysine 36, or other methylations in histone H3.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Histone H3 [Trimethyl Lys36] Antibody (RM155)
Western Blot: Histone H3 [Trimethyl Lys36] Antibody (RM155) [NBP2-61530] -
Western Blot: Histone H3 [Trimethyl Lys36] Antibody (RM155) [NBP2-61530] - Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using NBP2-61530 at 2 ug/ml. Observed molecular weight is ~16 kDa.
Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys36] Antibody (RM155) [NBP2-61530] - ChIP performed on HeLa cells using H3K36me3 antibody (NBP2-61530). Real-time PCR was performed using primers specific to the gene indicated.
Multiplex Immunoassay: Histone H3 [Trimethyl Lys36] Antibody (RM155) [NBP2-61530] - NBP2-61530 specifically reacts to Histone H3 trimethylated at Lysine 36 (K36me3). No cross reactivity with non-modified Lysine 36 (K36 Ctrl), monomethylated Lysine 36 (K36me1) or dimethylated Lysine 36 (K36me2), or other methylations in Histone H3.
Applications for Histone H3 [Trimethyl Lys36] Antibody (RM155)
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
2 ug/mL- 10 ug/mL
ELISA
0.2 ug/mL - 1 ug/ml
Multiplex Immunoassay
0.1 ug/ml - 0.5 ug/ml
Western Blot
0.5 ug/ml - 2 ug/ml
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
50% Glycerol/PBS, 1% BSA
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Histone H3
Histones are nuclear proteins responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Changes in chromatin structure play a large role in the regulation of transcription. The chromatin fibers are compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.
Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. Posttranslational modifications such as acetylation of core histones regulates gene expression, thus altering protein function and regulation (1). Histone H3 is primarily acetylated at lysines 9, 14, 18, and 23 and have a theoretical molecular weight of 15 kDa. Acetylation at lysine 9 and 14 appears to control histone deposition, chromatin assembly and active transcription. Methylation of arginine residues within histone H3 has also been linked to transcription regulation. Histone H3 has been linked to various types of cancer as a biomarker through the aberrant expression of histone deacetylase (HDAC) enzymes and changes to chromatins (2-4).
References
1. Zhang, Y. X., Akumuo, R. C., Espana, R. A., Yan, C. X., Gao, W. J., & Li, Y. C. (2018). The histone demethylase KDM6B in the medial prefrontal cortex epigenetically regulates cocaine reward memory. Neuropharmacology, 141, 113-125. doi:10.1016/j.neuropharm.2018.08.030
2. Nandakumar, V., Hansen, N., Glenn, H. L., Han, J. H., Helland, S., Hernandez, K, ...Meldrum, D. R. (2016). Vorinostat differentially alters 3D nuclear structure of cancer and non-cancerous esophageal cells. Sci Rep, 6, 30593. doi:10.1038/srep30593
3. Zhou, M., Li, Y., Lin, S., Chen, Y., Qian, Y., Zhao, Z., & Fan, H. (2019). H3K9me3, H3K36me3, and H4K20me3 Expression Correlates with Patient Outcome in Esophageal Squamous Cell Carcinoma as Epigenetic Markers. Dig Dis Sci, 64(8), 2147-2157. doi:10.1007/s10620-019-05529-2
4. Li, Y., Guo, D., Sun, R., Chen, P., Qian, Q., & Fan, H. (2019). Methylation Patterns of Lys9 and Lys27 on Histone H3 Correlate with Patient Outcome in Gastric Cancer. Dig Dis Sci, 64(2), 439-446. doi:10.1007/s10620-018-5341-8
Alternate Names
H3F3A, H3K36Me3
Gene Symbol
H3C14
Additional Histone H3 Products
Product Documents for Histone H3 [Trimethyl Lys36] Antibody (RM155)
Product Specific Notices for Histone H3 [Trimethyl Lys36] Antibody (RM155)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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