HIF-3 alpha Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2529

Discontinued Product
NB100-2529 has been discontinued. View all HIF-3 alpha/HIF3A products.

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all HIF-3 alpha/HIF3A Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an internal region of human HIF-3 alpha (within residues 250-350).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HIF-3 alpha Antibody

Western Blot: HIF-3 alpha Antibody [NB100-2529]

Western Blot: HIF-3 alpha Antibody [NB100-2529]

Western Blot: HIF-3 alpha Antibody [NB100-2529] - Detection of HIF-3 alpha using NB100-2529. Lane 1: Cos7 (-) control. Lane 2: Cos7 CoCl2 treated (+) control.
Immunocytochemistry/ Immunofluorescence: HIF-3 alpha Antibody [NB100-2529]

Immunocytochemistry/ Immunofluorescence: HIF-3 alpha Antibody [NB100-2529]

Immunocytochemistry/Immunofluorescence: HIF-3 alpha Antibody [NB100-2529] - HIF3 alpha antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 549 (red).
Western Blot: HIF-3 alpha Antibody [NB100-2529]

Western Blot: HIF-3 alpha Antibody [NB100-2529]

Western Blot: HIF-3 alpha Antibody [NB100-2529] - HIF-3a expression in papillary renal cell carcinoma samples. Image from verified customer review.

Applications for HIF-3 alpha Antibody

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunocytochemistry/ Immunofluorescence

1:100

Immunoprecipitation

1:10 - 1:500

Western Blot

1:500 - 1:1000
Application Notes
In Western Blot analysis, a band is recognized at ~68 kDa representing HIF-3 alpha. Because a band at ~71 kDa is also seen in hypoxic samples, it is important to run a negative control lysate as well (see image). In ICC/IF, cytoplasmic and nuclear staining was observed in HeLa cells, which is consistent with the literature under normoxic conditions for HIF-3 alpha (PMID: 16775626).

Reviewed Applications

Read 1 review rated 5 using NB100-2529 in the following applications:

Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Formulation

PBS

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: HIF-3 alpha/HIF3A

Hypoxia-inducible factor (HIF) is one of the most important factors in the cellular response to hypoxia, transcriptionally activating genes encoding proteins that mediate adaptive responses to reduced oxygen availability. HIF is a heterodimer consisting of one of three subunits, HIF1 alpha, HIF2 alpha, or HIF3 alpha. HIF target genes play critical roles in metabolism, angiogenesis, cell proliferation and cell survival. HIF3 alpha protein is one of several alpha/beta-subunit heterodimeric transcription factors that regulate many adaptive responses to low oxygen tension (hypoxia). The alpha 3 subunit lacks the transactivation domain found in factors containing either the alpha 1 or alpha 2 subunits. HIF3 alpha may be a marker for tumor growth and angiogenesis.

Long Name

Hypoxia Inducible Factor 3 Subunit Alpha

Alternate Names

BHLHe17, HIF3A, IPAS, MOP7, PASD7

Gene Symbol

HIF3A

Additional HIF-3 alpha/HIF3A Products

Product Documents for HIF-3 alpha Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for HIF-3 alpha Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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