GRAMD1A Antibody - BSA Free

Western Blot: GRAMD1A Antibody [NBP1-93730]

Western Blot: GRAMD1A Antibody [NBP1-93730] - Analysis in human cell lines A-549 and MCF-7 using anti-GRAMD1A antibody. Corresponding GRAMD1A RNA-seq data are presented for the same cell lines. Loading control: anti-HSP90B1.

Immunohistochemistry-Paraffin: GRAMD1A Antibody [NBP1-93730]

Immunohistochemistry-Paraffin: GRAMD1A Antibody [NBP1-93730] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.

Western Blot: GRAMD1A Antibody - BSA Free [NBP1-93730] -

Functional Characterization of GRAMD1A in WiT-49 Wilms Tumor Cells. (A) GRAMD1A expression levels in WiT-49 cells, demonstrating baseline expression prior to gene silencing. (B) Verification of GRAMD1A knockdown efficiency in WiT-49 cells transfected with GRAMD1A-specific siRNA (siGRAMD1A) or negative control siRNA (siNC). GRAMD1A mRNA levels were measured using RT-qPCR, while protein levels were assessed via Western blot analysis. (C) Colony formation assay showing the effects of GRAMD1A silencing on the proliferation capability of WiT-49 cells, highlighting reduced colony formation in GRAMD1A knockdown cells compared to siNC. (D) CCK-8 assay results depicting the effect of GRAMD1A knockdown on cell viability over time in WiT-49 cells. Cells transfected with siGRAMD1A exhibited significantly reduced viability compared to control cells, indicating GRAMD1A’s role in promoting cell proliferation. (E) Transwell migration and invasion assays used to assess the impact of GRAMD1A silencing on the migratory and invasive behavior of WiT-49 cells. GRAMD1A knockdown led to a marked reduction in both migration and invasion compared to the negative control group. These results collectively demonstrate the role of GRAMD1A in regulating the proliferation, migration, and invasion of WiT-49 cells, further supporting its potential as a therapeutic target in Wilms Tumor. (*p < 0.05, **p < 0.01, ***p < 0.001). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39659787), licensed under a CC-BY license. Not internally tested by Novus Biologicals.