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GRAIL/RNF128 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-24610

Novus Biologicals, part of Bio-Techne
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NBP2-24610
NBP2-24610SS

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Equine, Primate

Cited:

Human, Mouse

Predicted:

Bovine (94%), Chimpanzee (100%), Monkey (100%), Orangutan (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

Product Specifications

Immunogen

A synthetic peptide corresponding to amino acids portion of amino acids 227-269 of human GRAIL was used as immunogen, GenBank no. NP_919445.1.

Reactivity Notes

The amino acid sequence used as immunogen is 100% homologous in chimpanzee, monkey and orangutan, 94% homologous in cow, 88% homologous in cat and 83% homologous in opossum, rat and mouse.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GRAIL/RNF128 Antibody - BSA Free

Western Blot: GRAIL/RNF128 Antibody [NBP2-24610]

Western Blot: GRAIL/RNF128 Antibody [NBP2-24610]

GRAIL-RNF128-Antibody-Western-Blot-NBP2-24610-img0006.jpg
Immunohistochemistry-Paraffin: GRAIL/RNF128 Antibody [NBP2-24610]

Immunohistochemistry-Paraffin: GRAIL/RNF128 Antibody [NBP2-24610]

Immunohistochemistry-Paraffin: GRAIL/RNF128 Antibody [NBP2-24610] - Staining of normal human brain using control (rabbit Ig) at 5 ug/ml.
Western Blot: GRAIL/RNF128 Antibody [NBP2-24610]

Western Blot: GRAIL/RNF128 Antibody [NBP2-24610]

Western Blot: GRAIL/RNF128 Antibody [NBP2-24610] - Analysis of human GRAIL in human spleen lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse spleen and 4) rat spleen using NBP2-24610 at 1 ug/ml.

Applications for GRAIL/RNF128 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

reported in scientific literature (PMID 24356810)

Immunohistochemistry-Paraffin

5-10 ug/ml

Western Blot

0.5-2 ug/ml

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GRAIL/RNF128

The protein encoded by the RNF128 gene is a type I transmembrane protein that localizes to the endocytic pathway. This protein contains a RING zinc-finger motif and has been shown to possess E3 ubiquitin ligase activity. Expression of this gene in retrovirally transduced T cell hybridoma significantly inhibits activation-induced IL2 and IL4 cytokine production. Induced expression of this gene was observed in anergic CD4(+) T cells, which suggested a role in the induction of anergic phenotype. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq]

Long Name

Gene Related to Anergy in Lymphocytes Protein/Ring Finger Protein 128

Alternate Names

RNF128

Gene Symbol

RNF128

UniProt

Additional GRAIL/RNF128 Products

Product Documents for GRAIL/RNF128 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GRAIL/RNF128 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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