GRAF Antibody - BSA Free

Immunohistochemistry-Paraffin: GRAF Antibody [NBP1-89732]

Immunohistochemistry-Paraffin: GRAF Antibody [NBP1-89732] - Staining of human pancreas shows low expression as expected.

Western Blot: GRAF Antibody - BSA Free [NBP1-89732]

Analysis in human cell lines Caco-2 and MCF-7 using Anti-ARHGAP26 antibody. Corresponding ARHGAP26 RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.

Immunofluorescence: GRAF Antibody - BSA Free [NBP1-89732] -

Cdc42 and GRAF1 regulate the formation of tubular structures in activated T cells. (A) Jurkat T cells expressing GFP-GRAF1 (left) or GFP-GRAF1-BAR-PH (right) were activated on coverslips coated with functional antibodies against CD3 epsilon and CD28 for 10 min and fixed with paraformaldehyde (PFA). Images are maximum intensity projection of confocal z-stacks (B) Left: Maximum intensity projection of a Jurkat T cell expressing GFP-GRAF1 and myc-Cdc42-Q61L and activated as in (A). Right: the same cell shown in a 3D reconstruction. (C) Blinded quantification of the percentage of cells expressing GFP-GRAF1 and mCherry-tagged Cdc42 variants as indicated and having at least one GRAF1-positive tubule. Each data point represents the mean of an individual experiment, 75–136 cells per experiment. Small horizontal lines indicate mean (+/-SEM), * p < 0.05; *** p < 0.001; unpaired, two-tailed student’s t-test. (D) Jurkat T cells expressing GFP-Cdc42-Q61L, activated and fixed as in (A) and stained with an antibody against endogenous GRAF1. Arrows indicate GRAF1-positive tubules. Images representative of at least three independent experiments. Scale bar: 5 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31690048), licensed under a CC-BY license. Not internally tested by Novus Biologicals.