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Glutathione Peroxidase 3/GPX3 Antibody

Catalog # NBP1-06398 | Novus Biologicals a Bio-Techne Brand
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NBP1-06398
NBP1-06398SS

Key Product Details

Species Reactivity

Human, Rat, Primate

Applications

Immunoblotting, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Product Summary for Glutathione Peroxidase 3/GPX3 Antibody

Immunogen

Full length human Glutathione Peroxidase 3 expressed in E. coli. [Swiss-Prot# P22352]

Reactivity Notes

Immunogen sequence has 89% identity to mouse, 88% identity to bovine and 84% identity to porcine.

Localization

Secreted.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Glutathione Peroxidase 3/GPX3 Antibody

Immunohistochemistry: Glutathione Peroxidase 3/GPX3 Antibody [NBP1-06398] - GPX3 expressions in adenocarcinoma (AC). EnVision immunohistochemistry, original magnification x200. GPX3 positive reaction was mainly localized in the cytoplasm. Negative GPX3 expression in poorly differentiated AC (c), and positive GPX3 expression in well-differentiated AC (d). Image collected and cropped by CiteAb from the following publication (http://www.hindawi.com/journals/dm/2013/187043/), licensed under a CC-BY license.
Immunohistochemistry: Glutathione Peroxidase 3/GPX3 Antibody [NBP1-06398] - GPX3 expressions in squamous carcinomas/adenosquamous carcinomas (SC/ASC). EnVision immunohistochemistry, original magnification x200. GPX3 positive reaction was mainly localized in the cytoplasm. Negative GPX3 expression in poorly differentiated SC/ASC (c). Positive GPX3 expression in well-differentiated SC/ASC (d). Image collected and cropped by CiteAb from the following publication (http://www.hindawi.com/journals/dm/2013/187043/), licensed under a CC-BY license.
Western Blot: Glutathione Peroxidase 3/GPX3 Antibody [NBP1-06398] - Analysis of GPX3 in human kidney lysate.

Applications for Glutathione Peroxidase 3/GPX3 Antibody

Application
Recommended Usage

Immunoblotting

reported in scientific literature (PMID 29571702)

Immunocytochemistry/ Immunofluorescence

1:200

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

1:200. Use reported in scientific literature (PMID 24167362)

Western Blot

1:500
Application Notes
In Western blot, a band is seen ~25 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 9 publications using NBP1-06398 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Preservative

0.1% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Glutathione Peroxidase 3/GPX3

GPX3 (Glutathione Peroxidase 3) belongs to the glutathione peroxidase family. It protects cells and enzymes from oxidative damage by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide by glutathione. There is increasing evidence that GPX3 is a novel tumor suppressor and a therapeutic target for insulin resistance and diabetes mellitus.

Alternate Names

GPX-P, GPX3

Entrez Gene IDs

2878 (Human); 64317 (Rat)

Gene Symbol

GPX3

UniProt

Product Documents for Glutathione Peroxidase 3/GPX3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Glutathione Peroxidase 3/GPX3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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