Glucosylceramidase/GBA Antibody (JM10-76)

Western Blot Shows Glucosylceramidase/GBA Specificity Using Knockout Cell Line.

Western blot shows lysates of HAP1 parental cell line and Glucosylceramidase/GBA knockout HAP1 cell line (KO). Nitrocellulose membrane was probed with Glucosylceramidase/GBA Antibody (JM10-76) (Catalog # NBP2-66871) followed by HRP-conjugated secondary antibody. A specific band was detected for Glucosylceramidase/GBA at approximately 59.7 kDa (as indicated) in the parental HAP1 cell line, but is not detectable in knockout HAP1 cell line. Primary antibody dilution used: 1/500. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.

Western Blot: Glucosylceramidase/GBA Antibody (JM10-76) [NBP2-66871]

Western Blot: Glucosylceramidase/GBA Antibody (JM10-76) [NBP2-66871] - All lanes: Western blot analysis of GBA with anti-GBA antibody [JM10-76 at 1:1,000 dilution.Lane 1: Wild-type HEK293T whole cell lysate (20 ug).Lane 2: GBA knockout HEK293T whole cell lysate (20 ug). NBP2-66871was shown to specifically react with GBA in wild-type HEK293T cells. No band was observed when GBA knockout sample was tested. Wild-type and GBA knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (1/1,000) and Loading control antibody (Rabbit anti-GAPDH , 1/10,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry-Paraffin: Glucosylceramidase/GBA Antibody (JM10-76) [NBP2-66871]

Immunohistochemistry-Paraffin: Glucosylceramidase/GBA Antibody (JM10-76) [NBP2-66871] - Analysis of paraffin-embedded mouse pancreas tissue using anti-GBA antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.