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GFP Antibody

Catalog # NB600-308 | Novus Biologicals a Bio-Techne Brand

Key Product Details

Species Reactivity

Non-species specific

Applications

Dot Blot, ELISA, Electron Microscopy, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Free-Floating, Immunohistochemistry Whole-Mount, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for GFP Antibody

Immunogen

The immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.

Reactivity Notes

No reaction was observed against Human, Mouse serum proteins. Suitable for detecting fusion proteins containing the GFP sequence expressed in Human, Mouse, Rat, C. elegans, Drosophila and in vitro transcription/translation systems and transgenic animals. Known cross reactivity with wt and all variants such as rGFP, eGFP, S65T-GFP, RS-GFP, YFP and EGFP.

GFP Transgenic Rat reactivity reported in scientific literature (PMID:25724725).

Mouse reactivity reported in multiple pieces of scientific literature.

Transgenic C. elegans reactivity reported in scientific literature (PMID: 27110099).

Use in Mouse reported in secitific publication (PMID: 32765228).

Plant reactivity reported in scientific literature (PMID:32896843)

Specificity

No reaction was observed against Human, Mouse or Rat serum proteins.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria)

GFP antibody is stable for several weeks at 4C as an undiluted liquid.

Scientific Data Images for GFP Antibody

Immunocytochemistry/Immunofluorescence Detection of GFP in Transgenic Mouse Pancreas Cells

Immunocytochemistry/Immunofluorescence Detection of GFP in Transgenic Mouse Pancreas Cells

Analysis of GFP in transgenic mouse pancreas in OCT. The blue region in the center is an islet which does not express GFP and is negative. Image from verified customer review.
Western Blot Detection of GFP Tagged PKA Subunits in HEK293T Cells

Western Blot Detection of GFP Tagged PKA Subunits in HEK293T Cells

Analysis of GFP tagged PKA subunits over-expressed in HEK293T cells using GFP antibody. Image from verified customer review.
Immunohistochemical Staining of GFP+ Hepatocytes in Paraffin Embedded Liver

Immunohistochemical Staining of GFP+ Hepatocytes in Paraffin Embedded Liver

Staining of GFP+ hepatocytes (brown) transplanted into the liver of a recipient animal using anti-GFP antibody. Cyclin D1 double staining in blue. Image from verified customer review.

Applications for GFP Antibody

Application
Recommended Usage

ELISA

1:20000-1:120000

Electron Microscopy

1:10-1:500

Flow Cytometry

1:10-1:1000

Immunocytochemistry/ Immunofluorescence

1:500-1:5000

Immunohistochemistry

1:200-1:3000

Immunohistochemistry-Frozen

1:50-1:250

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:500-1:5000
Application Notes
This product is designed to detect GFP and its variants. GFP antibody has been tested by western blot and ELISA. This product can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. Biotin conjugated polyclonal anti-GFP used in a sandwich ELISA is well suited to titrate GFP in solution using this antibody in combination with monoclonal anti-GFP using either form of the antibody as the capture or detection antibodies. However, use the monoclonal form only for the detection of wild type or recombinant GFP as this form does not sufficiently detect 'enhanced' GFP. The detection antibody is typically conjugated to biotin and subsequently reacted with streptavidin conjugated HRP. Fluorochrome conjugated polyclonal anti-GFP can be used to detect GFP by immunofluorescence microscopy in prokaryotic (E.coli) and eukaryotic (CHO cells) expression systems and can detect GFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-GFP relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-GFP to detect GFP or GFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher.

Use in Immunoprecipitation reported in scientific literature (PMID:34887587).

Use in DB reported in scientific literature (PMID:34242364).

Use in Knockdown Validated reported in scientific literature
(PMID: 32905777).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 18 reviews rated 4.5 using NB600-308 in the following applications:

Published Applications

Read 265 publications using NB600-308 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GFP

Green fluorescent protein (GFP) is a 27 kDa protein derived from the bioluminescent jellyfish Aquorea Victoria. GFP can be excited by light in the blue to ultraviolet spectrum and has an excitation peak at 395 nm with an emission peak at 509 nm in the green spectrum. GFP autocatalytically forms a fluorescent pigment from natural amino acids present in the nascent protein. GFP is widely used as a reporter (tag) for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. GFP fluorescence is stable under fixation conditions and antibodies are suitable for a variety of applications including ELISA, ICC/IF, IHC, IP and WB (1-3).

References
1. Shi, C., Pan, F. C., Kim, J. N., Washington, M. K., Padmanabhan, C., Meyer, C. T., . . . Means, A. L. (2019). Differential Cell Susceptibilities to Kras(G12D) in the Setting of Obstructive Chronic Pancreatitis. Cell Mol Gastroenterol Hepatol. doi:10.1016/j.jcmgh.2019.07.001

2. Zhao, S., Fortier, T. M., & Baehrecke, E. H. (2018). Autophagy Promotes Tumor-like Stem Cell Niche Occupancy. Curr Biol, 28(19), 3056-3064.e3053. doi:10.1016/j.cub.2018.07.075

3. Zusso, M., Lunardi, V., Franceschini, D., Pagetta, A., Lo, R., Stifani, S., . . . Moro, S. (2019). Ciprofloxacin and levofloxacin attenuate microglia inflammatory response via TLR4/NF-kB pathway. J Neuroinflammation, 16(1), 148. doi:10.1186/s12974-019-1538-9

Long Name

Green Fluorescent Protein

Alternate Names

eGFP, GFPuv, anti-gfp (green fluorescent protein) pab, GFP immunofluorescence, GFP immunoprecipitation, GFP mouse, GFP staining, GFP western blot

UniProt

Product Documents for GFP Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GFP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for GFP Antibody

Showing  1 - 3 of 3 FAQs Showing All
  • Q: We are looking for a rabbit anti-GFP antibody as a primary antibody for immunofluorescence and WB. I've seen that you carry several anti-GFP antibodies and I would like to ask which one you would recommend. The one with Cat.No NB600-308 has been used in a lot of publications and I reckon it is a solid antibody. Is this our best choice, or is there a better alternative?

    A: NB600-308 is an excellent GFP antibody and I think it would work very well for you. It has been reviewed and published with which usually makes our customer feel much more confident, seeing as it worked in other people's hand.

  • Q: Does the GFP antibody NB600-308 also work against AcGFP (93% identical to EGFP) in IP and Western blot?

    A: For our product NB600-308, we have unfortunately not yet tested the product for cross reactivity with AcGFP. However, since the antibody is polyclonal and made to the full length AvGFP sequence, there is a good chance for cross reactivity.

  • Q: Hello, my lab purchased a GFP antibody from Novus in the past and I am now in need of an antibody to detect YFP and CFP as well. The GFP antibody we order has a part number of NB600-308. Would it possibly recognize YPF and CFP as well or will I need to purchase additional antibodies?

    A: NB600-308 is polyclonal to full length GFP, hence it will recognize most of its mutants. It will react with YFP and CFP.

Showing  1 - 3 of 3 FAQs Showing All
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