GCSH Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85842
![GCSH Antibody - BSA Free Western Blot: GCSH Antibody - BSA Free [NBP1-85842]](https://resources.bio-techne.com/images/products/nbp1-85842_rabbit-polyclonal-gcsh-antibody-24420259321574.jpg "Western Blot: GCSH Antibody - BSA Free [NBP1-85842]")
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot
Cited:
Simple Western
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: TLRTGPALLSVRKFTEKHEWVTTENGIGTVGISNFAQEALGDVVYCSLPEVGTKLNKQDEFGA
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for GCSH Antibody - BSA Free
![Immunocytochemistry/ Immunofluorescence: GCSH Antibody [NBP1-85842]](https://resources.bio-techne.com/images/products/GCSH-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85842-img0006.jpg "Immunocytochemistry/ Immunofluorescence: GCSH Antibody [NBP1-85842]")
Immunocytochemistry/ Immunofluorescence: GCSH Antibody [NBP1-85842]
Immunocytochemistry/Immunofluorescence: GCSH Antibody [NBP1-85842] - Staining of human cell line U-2 OS shows localization to vesicles. Antibody staining is shown in green.![Immunohistochemistry-Paraffin: GCSH Antibody [NBP1-85842]](https://resources.bio-techne.com/images/products/GCSH-Antibody-Immunohistochemistry-Paraffin-NBP1-85842-img0002.jpg "Immunohistochemistry-Paraffin: GCSH Antibody [NBP1-85842]")
Immunohistochemistry-Paraffin: GCSH Antibody [NBP1-85842]
Immunohistochemistry-Paraffin: GCSH Antibody [NBP1-85842] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules..![Western Blot: GCSH Antibody [NBP1-85842]](https://resources.bio-techne.com/images/products/GCSH-Antibody-Western-Blot-NBP1-85842-img0004.jpg "Western Blot: GCSH Antibody [NBP1-85842]")
Western Blot: GCSH Antibody [NBP1-85842]
Western Blot: GCSH Antibody [NBP1-85842] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251MG sp
Lane 4: Human plasma (IgG/HSA depleted)
Lane 5: Human liver tissue
Applications for GCSH Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Simple Western
1:50
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: GCSH
Alternate Names
GCE, glycine cleavage system H protein, mitochondrial, glycine cleavage system protein H (aminomethyl carrier), lipoic acid-containing protein, mitochondrial glycine cleavage system H-protein, NKH
Gene Symbol
GCSH
Additional GCSH Products
Product Documents for GCSH Antibody - BSA Free
Product Specific Notices for GCSH Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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