FTO Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB110-60935

Novus Biologicals, part of Bio-Techne
Discontinued Product
NB110-60935 has been discontinued. View all FTO products.

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Chicken, Primate

Cited:

Human, Avian - Chicken

Applications

Validated:

Immunohistochemistry, Western Blot, Immunoblotting

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.1 mg/ml

View all FTO Antibodies »

Product Specifications

Immunogen

A synthetic peptide within the C-terminal region (residues 400-505) of the human FTO protein. [Swiss-Prot# Q9C0B1]

Reactivity Notes

Chicken reactivity reported in scientific literature (PMID: 22019092).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FTO Antibody

Western Blot: FTO Antibody [NB110-60935]

Western Blot: FTO Antibody [NB110-60935]

Western Blot: FTO Antibody [NB110-60935] - Detection of FTO in human brain lysate using anti-FTO NB110-60935.

Applications for FTO Antibody

Application
Recommended Usage

Immunoblotting

reported in scientific literature (PMID 22019092)

Immunohistochemistry

reported in scientific literature (PMID 28205605)

Western Blot

0.5 ug/ml
Application Notes
In Western blot analysis, a band is seen at ~60 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.1% Sodium Azide

Concentration

1.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FTO

FTO (alpha-ketoglutarate-dependent dioxygenase FTO) is a novel protein belonging to FTO family and it is a nuclear dioxygenase that repairs alkylated DNA and RNA by oxidative demethylation reactions. FTO exerts highest demethylase activity towards single-stranded RNA containing 3-methyluracil, followed by single-stranded DNA containing 3-methylthymine, low activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine and is inactive towards 1-methylguanine or double-stranded DNA. FTO requires O2, alpha-ketoglutarate and iron (co-factor) for its activity and gets activated by ascorbate whereas its inhibitors include N-oxalylglycine, fumarate, succinate etc. It contributes to the regulation of global metabolic rate, energy expenditure/homeostasis and has been suggested to control the body size as well as accumulation of body fat. FTO has been associated with various obesity traits, insulin resistance and NIDDM, and its defects are known to cause growth retardation developmental delay coarse facies and early death (GDFD).

Long Name

Fat Mass and Obesity Associated

Alternate Names

Alpha-ketoglutarate-dependent dioxygenase FTO

Gene Symbol

FTO

Additional FTO Products

Product Documents for FTO Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for FTO Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.