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Flavivirus group antigen Antibody (D1-4G2-4-15 (4G2)) - Azide and BSA Free

Catalog # NBP2-52709 | Novus Biologicals a Bio-Techne Brand
Recombinant Monoclonal Antibody

Key Product Details

Species Reactivity

Virus

Applications

CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Neutralization, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG2a Kappa Clone # D1-4G2-4-15 (4G2)

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Summary

Immunogen

This recombinant Flavivirus group antigen Antibody (D1-4G2-4-15 (4G2)) was prepared from Dengue Virus type 2 antigens.

Reactivity Notes

Dengue Virus, Zika Virus, West Nile Virus, Yellow Fever Virus, Flaviviridae.

Specificity

This Flavivirus group antigen Antibody (D1-4G2-4-15 (4G2)) recognises flavivirus group specific antigens (Dengue virus, West Nile Virus, Japanese Encephalitis, Yellow Fever Virus, Zika virus etc). It binds to the fusion loop at the extremity of domain II of protein E.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images

Immunofluorescent Detection of Flavivirus group antigen in HEK 293 Cells Infected with Dengue Virus and Inhibitors

Immunofluorescent Detection of Flavivirus group antigen in HEK 293 Cells Infected with Dengue Virus and Inhibitors

IF assay in a high throughput format for screening Dengue virus inhibitors. HEK 293 cells in DMEM 5% FBS/1% PS at 5000 cells/well are infected in presence of inhibitors with Dengue 2 New Guinea (VR-1584) using 384-well collagen-coated plates. After 48 h incubation at 37C/5% CO2, the supernatant is removed and the Alexa Fluor 647 conjugated Ab is added at 1:5250. Plates were read after O/N incubation. Columns 1-2: Cells control. 23-24, rows A-L: Cells + virus control. 23-24, rows M-P: Cells + virus control + control drug (Positive control). 3-12 and 13-22: Experimental compounds added in a dose response manner from high to low concentration (Columns 3-13 higher concentration, columns 12-22 lower concentration). Image using the Alexa Fluor 647 format of this antibody.
Flow Cytometry of Infected and Uninfected C3/36 Cells Using Flavivirus group antigen Antibody

Flow Cytometry of Infected and Uninfected C3/36 Cells Using Flavivirus group antigen Antibody

Human C3/36 cell line. Flow cytometry image submitted by a verified customer review.
Flow Cytometry of Zika Virus Infected Mk2 Cells Stained with Flavivirus group antigen Antibody

Flow Cytometry of Zika Virus Infected Mk2 Cells Stained with Flavivirus group antigen Antibody

Flow Cytometry: Flavivirus group antigen Antibody (D1-4G2-4-15 (4G2)) [PE] [NBP2-52709PE] - Mk2 cell line was infected with ZIKA virus at 72 p.o.i and the sample was stained and analyzed by flow cytometry. Image from verified customer review. Image using the PE form of this antibody.

Applications

Application
Recommended Usage

ELISA

1:100 - 1:2000

Flow Cytometry

1:10 - 1:1000

Immunofluorescence

1:10 - 1:500

Western Blot

1:100 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 22 publications using NBP2-52709 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: Flavivirus group antigen

Flaviviruses are a group of seventy enveloped, positive-sense single stranded RNA viruses belonging to the Flaviviridae family (1-5). Flaviviruses are typically transmitted through arthropods, like mosquitoes and ticks, and can infect humans causing outbreaks and serious illness or death worldwide (1-5). These well-known viral pathogens include dengue virus (DENV), West Nile virus (WNV), Japanese encephalitis virus (JEV), tick-borne encephalitis virus (TBEV), Yellow Fever virus (YFV), and Zika virus (ZIKV) (1-5). Flaviviruses consist of ~10.7 kb RNA genome with one open reading frame (ORF) that is flanked by 5' and 3' untranslated regions (UTRs) (1, 2, 6). The ORF encodes for a polyprotein that is made up of three structural proteins, the capsid (C), premembrane (prM), and envelope (E) proteins, and seven non-structural (NS) proteins (NS1, NS2A/B, NS3, NS4A, 2K, NS4B, and NS5) (1, 2, 5, 6).

The Flavivirus group antigen antibody binds to the fusion loop at the distal end of domain II (EDII) of protein E. Flavivirus E is a glycoprotein that is the main antigen for membrane fusion between virus and host and entry into the cell, as well as inducing protective immunity by stimulating neutralizing antibodies (2, 4, 6). The E protein is 495 amino acids (aa) in length and has a theoretical molecular weight between 55 - 72 kDa, which varies based on glycosylation sites (2). Flavivirus E protein contains three domains (EDI, EDII, and EDIII) followed by a stem that contents to the helix-transmembrane domains (TMDs, TM1 and TM2) (2, 4, 6). The EDII contains an S-S bridge at its distal end and functions as a fusion loop (FL) for virus-mediated membrane fusion and antibody binding (2). Given the important role of Flavivirus protein E in host cell infection and membrane fusion, it has become a target for flavivirus disease prevention and treatment (2, 5).

Alternative names for Flavivirus group antigen include Envelope Protein and Protein E.

References

1. Ngono, A. E., & Shresta, S. (2018). Immune Response to Dengue and Zika. Annual review of immunology. https://doi.org/10.1146/annurev-immunol-042617-053142

2. Rey, F. A., Stiasny, K., Vaney, M. C., Dellarole, M., & Heinz, F. X. (2018). The bright and the dark side of human antibody responses to flaviviruses: lessons for vaccine design. EMBO reports. https://doi.org/10.15252/embr.201745302

3. Zhang, X., Jia, R., Shen, H., Wang, M., Yin, Z., & Cheng, A. (2017). Structures and Functions of the Envelope Glycoprotein in Flavivirus Infections. Viruses. https://doi.org/10.3390/v9110338

4. Laureti, M., Narayanan, D., Rodriguez-Andres, J., Fazakerley, J. K., & Kedzierski, L. (2018). Flavivirus Receptors: Diversity, Identity, and Cell Entry. Frontiers in immunology. https://doi.org/10.3389/fimmu.2018.02180

5. Boldescu, V., Behnam, M., Vasilakis, N., & Klein, C. D. (2017). Broad-spectrum agents for flaviviral infections: dengue, Zika and beyond. Nature reviews. Drug discovery. https://doi.org/10.1038/nrd.2017.33

6. Crill, W. D., & Chang, G. J. (2004). Localization and characterization of flavivirus envelope glycoprotein cross-reactive epitopes. Journal of virology, 78(24), 13975-13986. https://doi.org/10.1128/JVI.78.24.13975-13986.2004

Alternate Names

Envelope Protein, Protein E

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs

Showing  1 - 1 of 1 FAQ Showing All
  • Q: What is your recommended dilution for this antibody  in Flow Cytometry application?

    A: We normally will not have dilutions listed for the conjugated antibodies, only the applications.
    We would refer you to the unconjugated antibody for the recommended dilution for an application

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