FGFR3 Antibody (OTI1B10) [CoraFluor™ 1]
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70722CL1
![FGFR3 Antibody (OTI1B10) [CoraFluor™ 1]](https://resources.bio-techne.com/images/products/nbp2-70722cl1_mouse-fgfr3-mab-oti1b10-corafluor-1-249202516354514.png "Product Feature: CoraFluor Probes for TR-FRET")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
CoraFluor 1
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI1B10
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Human recombinant protein fragment corresponding to amino acids 23-375 of human FGFR3 (NP_000133) produced in SF9 cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
CoraFluor(TM) 1, amine reactive
CoraFluor(TM) 1, thiol reactive
For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
CoraFluor(TM) 1, amine reactive
CoraFluor(TM) 1, thiol reactive
For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
Scientific Data Images for FGFR3 Antibody (OTI1B10) [CoraFluor™ 1]
Product Feature: CoraFluor Probes for TR-FRET
CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.Applications for FGFR3 Antibody (OTI1B10) [CoraFluor™ 1]
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: FGFR3
FGFR3 (fibroblast growth factor receptor 3), also known as CEK2 and CD333, is a transmembrane receptor tyrosine kinase that plays a role in skeletal development and tumorigenesis. Alternative splicing generates beta isoforms of the receptor which lack the first Ig-like domain as well as IIIb isoforms that have a substitution in the third Ig-like domain. FGFR3 (IIIb) triggers cell proliferation in response to FGF acidic and FGF-9, while FGFR3 (IIIc) shows a wider selectivity that includes FGF acidic, FGF basic/FGF2, FGF-4, -8, -9, -17, -18, -19, and -20. FGF mediated activation of FGFR3 is dependent on the presence of heparan sulfate proteoglycans. FGFR3 functions as a negative regulator of endochondral bone growth, and FGFR3 mutations are associated with chondrodysplasia in humans. The development of many cancers is associated with mutations or dysregulation of FGFR3.
Long Name
Fibroblast Growth Factor Receptor 3
Alternate Names
CD333, CEK, FGF R3, JTK4
Gene Symbol
FGFR3
Additional FGFR3 Products
Product Documents for FGFR3 Antibody (OTI1B10) [CoraFluor™ 1]
Product Specific Notices for FGFR3 Antibody (OTI1B10) [CoraFluor™ 1]
CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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