F4/80 Antibody (BM8) [FITC]
Novus Biologicals, part of Bio-Techne | Catalog # NB100-77700
Discontinued Product
NB100-77700 has been discontinued.
View all F4/80 products.
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Human, Mouse
Applications
Validated:
Flow Cytometry
Cited:
Flow Cytometry
Label
FITC (Excitation = 495 nm, Emission = 519 nm)
Antibody Source
Monoclonal Rat IgG2A Clone # BM8
Concentration
0.5 mg/ml
Product Specifications
Immunogen
This F4/80 Antibody (BM8) was developed against BALB/c macrophages obtained from 14-day-old bone marrow cell cultures.
Reactivity Notes
The monoclonal antibody BM8 cross reacts with human heart macrophages.
Marker
Macrophage Marker
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Applications for F4/80 Antibody (BM8) [FITC]
Application
Recommended Usage
Flow Cytometry
< = 0.25 ug/10^6 cells in 100 ul
Application Notes
This antibody works in Flow Cytometry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot. The typical starting working dilution is 1:50. F4/80 is sensitive to 2-mercaptoethanol. The antibody requires proteinase K treatment of paraffin sections.
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS (pH 7.2)
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: F4/80
Depending on factors such as differentiation, activation, and location, the level of F4/80 expression can vary between cell-types (1,2). For example, peritoneal macrophages are subdivided based on morphology and expression with large peritoneal macrophages having high levels of F4/80 and small peritoneal macrophages having low F4/80 expression (1). Furthermore, splenic macrophages are classified based on location (red pulp, marginal zone, and white pulp) and F4/80 expression level varies within these populations as well; the red pulp macrophages have high levels of F4/80 and CD68 by comparison (1). Originally, the F4/80 monoclonal antibody was generated for a macrophage-specific surface antigen, however F4/80 staining is also observed in other hematopoietic cell populations, thus other immune cell markers should be used in conjunction with F4/80 to distinguish between different populations (1). Though the full extent of F4/80 ligands and biological functions remains elusive, studies have suggested that F4/80 is critical for macrophage-natural killer cell interaction and cytokine release (1,2). Additionally, a study of anterior chamber-associated immune deviation (ACAID) indicated a possible role for F4/80 in the generation of regulatory T cells (1,2).
References
1. Dos Anjos Cassado A. (2017). F4/80 as a Major Macrophage Marker: The Case of the Peritoneum and Spleen. Results and Problems in Cell Differentiation. https://doi.org/10.1007/978-3-319-54090-0_7
2. Hamann, J., Hsiao, C. C., Lee, C. S., Ravichandran, K. S., & Lin, H. H. (2016). Adhesion GPCRs as Modulators of Immune Cell Function. Handbook of Experimental Pharmacology. https://doi.org/10.1007/978-3-319-41523-9_15
Alternate Names
DD7A5-7, Gpf480, Ly71
Gene Symbol
Adgre1
Additional F4/80 Products
Product Documents for F4/80 Antibody (BM8) [FITC]
Product Specific Notices for F4/80 Antibody (BM8) [FITC]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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