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ER alpha/NR3A1 Antibody (33)

Catalog # NB300-560 | Novus Biologicals a Bio-Techne Brand
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NB300-560
NB300-560-0.025mg

Key Product Details

Species Reactivity

Human, Mouse, Rat, Avian, Bovine, Gerbil, Primate

Applications

Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 33

Concentration

1 mg/ml

Product Summary for ER alpha/NR3A1 Antibody (33)

Immunogen

Synthetic peptide corresponding to the residues E(247) V G M M K G G I R K D R R G(261) of the ER alpha DNA binding domain.

Reactivity Notes

Gerbil reactivity reported in scientific literature (PMID: 31753310).

Localization

Nuclear

Specificity

Detects both the steroid occupied and unoccupied forms of the estrogen receptor (ER) alpha. This does not cross react with ER beta, androgen receptor, progesterone receptor, or glucocorticoid receptor.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

67 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

**For Western blot QC, 50 ug bovine liver was analyzed. Blocking was in 5% milk in PBST for 1 hour. NB300-560 diluted to 1ug/ml was incubated with blocking buffer for 1 hour RT. Three x 8 minute washes were performed, then secondary antibody added, repeat washes, then ECL reagents used. Film exposure determined positive signal.

Scientific Data Images for ER alpha/NR3A1 Antibody (33)

Western Blot: ER alpha/NR3A1 Antibody (33) [NB300-560] - Analysis of recombinant ER alpha.
Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody (33) [NB300-560] - Human breast tissue (B) and magnified section (C) compared with an isotype control (A).
Immunohistochemistry-Frozen: ER alpha/NR3A1 Antibody (33) [NB300-560] - Human breast carcinoma (B) and magnified section (C) compared with an isotype control (A).

Applications for ER alpha/NR3A1 Antibody (33)

Application
Recommended Usage

Immunofluorescence

10 ug/ml

Immunohistochemistry

1:20 - 1:100

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

4 ug/ml
Application Notes
Blocking usage was reported in scientific literature (PMID: 2360217).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 1 using NB300-560 in the following applications:

Published Applications

Read 10 publications using NB300-560 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

Dulbecco's PBS with 1 mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ER alpha/NR3A1

Estrogen Receptor alpha is a 65 kDa protein and a member of the steroid family of nuclear receptors. Estrogen Receptor alpha is a ligand-activated transcription factor that, when bound to estrogen, induces a conformational change that allows dimerization and binding to estrogen response element sequences. When bound to DNA, Estrogen Receptor alpha can positively or negatively regulate gene transcription. Like other steroid hormone receptors, Estrogen Receptors are intracellular proteins. Estrogen Receptors play an important role in regulating mammary gland growth and differentiation. Breast tumors that are ER positive may also show a more favorable response to anti-estrogen therapies

Long Name

Estrogen Receptor alpha

Alternate Names

ESR1, NR3A1

Entrez Gene IDs

2099 (Human); 13982 (Mouse); 24890 (Rat); 407238 (Bovine)

Gene Symbol

ESR1

UniProt

Product Documents for ER alpha/NR3A1 Antibody (33)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ER alpha/NR3A1 Antibody (33)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for ER alpha/NR3A1 Antibody (33)

Showing  1 - 2 of 2 FAQs Showing All
  • Q: I am interested in getting antibodies for ER (estrogen receptor) that would work in flow cytometry. Could you recommend some choices?

    A: NB300-560 and NBP2-19005 are both validated for detected estrogen receptor in flow cytometry. There are alpha and beta subunits of ER, I am assuming you are looking for the alpha subunit and this is what these antibodies are directed towards.

  • Q: Do you have data using this clone for flow cytometry? Do you have direct-conjugates for this antibody?

    A: This antibody has been tested/validated in many applications, including WB, B/N, Flow, GS, ICC/IF, IHC, IHC-P, and IP with a variety of species (Human, Mouse, Rat, Bovine, and Primate); and we guarantee 100% that this antibody will produce the positive results in the validated applications with the tested species. Or we will refund/free replace the antibody. Unfortunately, the lab where the flow experiments were done did not provide us any data, or we would have posted on the webpage. FYI, since it is not uncommon for the hybridoma cell lines, which the respective monoclonal antibodies are produced from, to be licensed out by the inventors' labs, you could find the same ER alpha/NR3A1 Antibody clone# 33, supplied by another supplier, was used in flow with human samples (PMID: 11002266). Since they are the identical antibody clone, the flow data could be the reference fot you if wanting to use NB300-560 in you ongoing flow experiments.

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