Enterovirus 71 VP1 Antibody (HL1928) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25450
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Virus
Applications
Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL1928 expressed in HEK293
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fragment of Enterovirus 71 VP1
Reactivity Notes
Enterovirus 71
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit Enterovirus 71 VP1 Antibody (HL1928) - Azide and BSA Free (NBP3-25450) is a recombinant monoclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Enterovirus 71 VP1 Antibody (HL1928) - Azide and BSA Free
Immunocytochemistry/Immunofluorescence: Enterovirus 71 VP1 Antibody (HL1928) - Azide and BSA Free [NBP3-25450] -
Immunocytochemistry/Immunofluorescence: Enterovirus 71 VP1 Antibody (HL1928) - Azide and BSA Free [NBP3-25450] - Enterovirus 71 VP1 antibody [HL1928] detects Enterovirus 71 VP1 protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Enterovirus 71 VP1 stained by Enterovirus 71 VP1 antibody [HL1928] (NBP3-25450) diluted at 1:500. Blue: Fluoroshield with DAPI .
ELISA: Enterovirus 71 VP1 Antibody (HL1928) - Azide and BSA Free [NBP3-25450] -
ELISA: Enterovirus 71 VP1 Antibody (HL1928) - Azide and BSA Free [NBP3-25450] - Sandwich ELISA detection of recombinant full-length Enterovirus 71 VP1 protein using antibodies as below. Capture: Enterovirus 71 VP1 antibody [HL1929] (NBP3-25451) (5 ug/mL) Detection: HRP-conjugated Enterovirus 71 VP1 antibody [HL1928] (NBP3-25450) (1 ug/mL) Please notice that NBP3-25450 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-25451. Please contact us for custom HRP-conjugated antibody.
Western Blot: Enterovirus 71 VP1 Antibody (HL1928) - Azide and BSA Free [NBP3-25450] -
Western Blot: Enterovirus 71 VP1 Antibody (HL1928) - Azide and BSA Free [NBP3-25450] - Non-infected (-) and infected (+) RD whole cell extracts (5 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus 71 VP1 antibody [HL1928] (NBP3-25450) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Applications for Enterovirus 71 VP1 Antibody (HL1928) - Azide and BSA Free
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Sandwich ELISA
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Enterovirus 71 VP1
Alternate Names
Capsid protein VP1, EV71 VP1, P1D, Virion protein 1
Additional Enterovirus 71 VP1 Products
Product Documents for Enterovirus 71 VP1 Antibody (HL1928) - Azide and BSA Free
Product Specific Notices for Enterovirus 71 VP1 Antibody (HL1928) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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