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Dtk/TYRO3 Antibody - BSA Free, Novus Biologicals

Bio-Techne includes Novus Biologicals | Catalog # NBP1-28635

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Rat (94%). Backed by our 100% Guarantee.

Applications

Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.19 mg/ml

Product Summary for Dtk/TYRO3 Antibody - BSA Free

Immunogen

Synthetic peptide made to an internal portion of the human TYRO3 protein (within residues 100-250). [Swiss-Prot# Q06418]

Reactivity Notes

Immunogen sequence has 94% identity to rat.

Localization

Cell membrane; Single-pass type I membrane protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

96 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Dtk/TYRO3 Antibody - BSA Free

Western Blot: Dtk/TYRO3 Antibody [NBP1-28635] - Lane 1: Western blot on normal human brain, Lane 2: Western blot on normal mouse brain.
Immunocytochemistry/Immunofluorescence: Dtk/TYRO3 Antibody [NBP1-28635] - Staining of HELA cells using NBP128635 (Green) at a 1:200 dilution detected using Dylight-488 conjugated goat anti-rabbit secondary antibody. Nuclei were visualized using Hoechst 33258 (Blue).
Flow Cytometry: Dtk/TYRO3 Antibody [NBP1-28635] - An intracellular stain was performed on HeLa cells with Dtk/TYRO3 Antibody NBP1-28635AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Applications for Dtk/TYRO3 Antibody - BSA Free

Application
Recommended Usage

Flow Cytometry

reported in scientific literature (PMID 27034374)

Immunocytochemistry/ Immunofluorescence

1:200

Western Blot

2 ug/ml
Application Notes
In Western blot, a band can be seen at ~96 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 4 publications using NBP1-28635 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1.19 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Tyro3/Dtk

TYRO3 (tyrosine-protein kinase receptor TYRO3) is a RTK which facilitate transduction of signals from extracellular matrix (ECM) into cytoplasm via its direct interaction with multiple ligands such as TUB, TULP1 or GAS6 for the regulation of multiple biological processes such as cell survival, migration and differentiation. TYRO3-ligand binding stimulates its dimerization as well as autophosphorylation on its intracellular domain that provides docking sites for the molecules of downstream signaling. Once stimulated, TYRO3 interacts with PIK3R1 leading to increased PI3K activity followed by AKT signaling activation including NFkB nuclear translocation mediated up-regulation of transcription of NFkB's target genes. TYRO3 signaling events are critical to neuron protection from excitotoxic injury, platelet aggregation, cytoskeletal reorganization, inhibition of TLRs-mediated innate immune response via STAT1 activation followed by production of cytokine signaling suppressors (SOCS1 and SOCS3). TYRO3 is highly expressed during CNS neurogenesis with distinct patterns in adult brain and has been closely related to CNS immunodysfunction.

Long Name

Tyrosine Protein Kinase Receptor TYRO3

Alternate Names

Brt, Dtk, Rse, Sky

Entrez Gene IDs

7301 (Human)

Gene Symbol

TYRO3

UniProt

Additional Tyro3/Dtk Products

Product Documents for Dtk/TYRO3 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Dtk/TYRO3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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