DNA Polymerase delta, catalytic subunit Antibody (JE35-73)

Western Blot: DNA Polymerase delta, catalytic subunit Antibody (JE35-73) [NBP3-32838]

Western Blot: DNA Polymerase delta, catalytic subunit Antibody (JE35-73) [NBP3-32838] - Western blot analysis of DNA Polymerase delta, catalytic subunit on different lysates with Rabbit anti-DNA Polymerase delta, catalytic subunit antibody (NBP3-32838) at 1/1,000 dilution.

Lane 1: HeLa cell lysate (20 ug/Lane)
Lane 2: A549 cell lysate (20 ug/Lane)
Lane 3: 293T cell lysate (20 ug/Lane)
Lane 4: Jurkat cell lysate (20 ug/Lane)
Lane 5: RAW264.7 cell lysate (20 ug/Lane)
Lane 6: PC-3M cell lysate (20 ug/Lane)
Lane 7: Mouse testis tissue lysate (40 ug/Lane)
Lane 8: Rat testis tissue lysate (40 ug/Lane)
Lane 9: SW480 cell lysate (20 ug/Lane)

Predicted band size: 124 kDa
Observed band size: 124 kDa

Exposure time: 1 minute;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32838) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: DNA Polymerase delta, catalytic subunit Antibody (JE35-73) [NBP3-32838]

Immunohistochemistry: DNA Polymerase delta, catalytic subunit Antibody (JE35-73) [NBP3-32838] - Immunohistochemical analysis of paraffin-embedded
rat testis tissue with Rabbit anti-DNA Polymerase delta, catalytic subunit antibody (NBP3-32838) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32838) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.