Dityrosine Antibody (7D4) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59360
![Western Blot: Dityrosine Antibody (7D4) [NBP2-59360]](https://resources.bio-techne.com/images/products/Dityrosine-Antibody-7D4-Western-Blot-NBP2-59360-img0006.jpg "Western Blot: Dityrosine Antibody (7D4) [NBP2-59360]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Non-species specific
Applications
Validated:
Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 7D4
Format
BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Synthetic Dityrosine conjugated to Keyhole Limpet Hemocyanin (KLH).
Specificity
Specific for dityrosine modified proteins. Does not cross-react with 3,5-dibromotyrosine or bromotyrosine modified proteins.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
Novus Biologicals Mouse Dityrosine Antibody (7D4) - BSA Free (NBP2-59360) is a monoclonal antibody validated for use in WB, ELISA and ICC/IF. Anti-Dityrosine Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Dityrosine Antibody (7D4) - BSA Free
Western Blot: Dityrosine Antibody (7D4) [NBP2-59360]
Western Blot: Dityrosine Antibody (7D4) [NBP2-59360] - Western Blot analysis of Dityrosine-BSA Conjugate showing detection of 67 kDa Dityrosine protein using Mouse Anti-Dityrosine Monoclonal Antibody, Clone 7D4 (NBP2-59360). Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA. Lane 3: 3,5-Dibromotyrosine-BSA. Lane 4: Dityrosine-BSA. Lane 5: Bromotyrosine-BSA. Lane 6: 7-ketocholesterol-BSA. Load: 1 ug. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Dityrosine Monoclonal Antibody (NBP2-59360) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.![Immunocytochemistry/ Immunofluorescence: Dityrosine Antibody (7D4) [NBP2-59360]](https://resources.bio-techne.com/images/products/Dityrosine-Antibody-7D4-Immunocytochemistry-Immunofluorescence-NBP2-59360-img0005.jpg "Immunocytochemistry/ Immunofluorescence: Dityrosine Antibody (7D4) [NBP2-59360]")
Immunocytochemistry/ Immunofluorescence: Dityrosine Antibody (7D4) [NBP2-59360]
Immunocytochemistry/Immunofluorescence: Dityrosine Antibody (7D4) [NBP2-59360] - Tissue: Embryonic kidney cells (HEK293) cultured overnight with 50 uM H2O2. Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Dityrosine Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Localization: Cytoplasmic. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) Dityrosine Antibody (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.Applications for Dityrosine Antibody (7D4) - BSA Free
Application
Recommended Usage
ELISA
1:1000
Immunocytochemistry/ Immunofluorescence
1:50
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.4), 50% Glycerol
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: Dityrosine
Alternate Names
Dityrosine;Dityrosine (DT);DT;DY
Additional Dityrosine Products
Product Documents for Dityrosine Antibody (7D4) - BSA Free
Product Specific Notices for Dityrosine Antibody (7D4) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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