Disialoganglioside GD3 Antibody (MB3.6) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81131
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG3 Clone # MB3.6
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
FM9 melanoma cells.
Specificity
Reacts specifically with human GD3 ganglioside (Cheresh et al., 1984). No cross-reactivity with other gangliosides purified from neuroblastoma or melanoma cells.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG3
Applications for Disialoganglioside GD3 Antibody (MB3.6) - BSA Free
Application
Recommended Usage
Flow Cytometry
1-2 ug per 10^6 cells
Application Notes
Immunohistochemistry (frozen tissue sections) Immunofluorescence of cells in culture. Flow cytometry: Lysis of GD3 positive (melanoma) cells in presence of complement (Cheresh et al., 1985). Induction of antibody dependent cellular cyotoxicity (ADCC) {Ortaldo JR et al 1987 J Immunol 138(10):3566-3572. TLC analysis: Sjoberg, ER (1995) J. Biol. Chem. 270(7):2921-2930.{http://www.jbc.org/cgi/content/full/270/7/2921}.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
0.02 M Phosphate Buffer (pH 7.6), 0.25 M NaCl
Format
BSA Free
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Disialoganglioside GD3
Alternate Names
3g11
Additional Disialoganglioside GD3 Products
Product Documents for Disialoganglioside GD3 Antibody (MB3.6) - BSA Free
Product Specific Notices for Disialoganglioside GD3 Antibody (MB3.6) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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