DEP-1/CD148 Antibody (A3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-45155

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry, Immunohistochemistry-Paraffin

Cited:

Flow Ctyometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # A3

Concentration

1.0 mg/ml

View all DEP-1/CD148 Antibodies »

Product Specifications

Immunogen

PHA-stimulated human PBMC

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for DEP-1/CD148 Antibody (A3)

DEP-1/CD148 Antibody (A3) Detection of CD148 in Human Tonsil.

Detection of CD148 in Human Tonsil.

CD148 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human CD148 Monoclonal Antibody (Catalog #NBP3-45155) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane of tonsil lymphocytes.
DEP-1/CD148 Antibody (A3) Detection of DEP-1/CD148 (A3) in Human PBMC lymphocytes by Flow Cytometry.

Detection of DEP-1/CD148 (A3) in Human PBMC lymphocytes by Flow Cytometry.

Human peripheral blood mononuclear cell (PBMC) lymphocytes were stained with Mouse Anti-Human CD148 Monoclonal Antibody (Catalog # NBP3-45155, filled histogram) or matched isotype control antibody (open histogram).

Applications for DEP-1/CD148 Antibody (A3)

Application
Recommended Usage

Flow Cytometry

0.5 - 2.0 ug/ml

Immunohistochemistry-Paraffin

1:100 - 1:500

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DEP-1/CD148

CD148 (also known as HPTP-eta or DEP-1) is a transmembrane protein tyrosin phosphatase containing eight fibronectin type III extracellular domains. This protein is known to inhibit transduction of mitogenic signals in non-hematopoietic cells (fibroblasts, epithelial cells), and signal transduction downstream of T cell receptor, however, it also augments immunoreceptor signaling in B cells and macrophages via dephosphorylating C-terminal tyrosine of Src-family tyrosine kinases. CD148 expression increases after in vitro activation of peripheral blood leucocytes. It can be also used as marker of the most mature human thymocytes, and leukemic cells corresponding to this stadium of thymocyte differentiation. In contrast, in mice the CD148 expression sharply drops through the double positive stage to the single positive thymocytes.

Long Name

Density Enhanced Receptor Protein Tyrosine Phosphatase

Alternate Names

CD148, DEP1, HPTP-eta, PTPRJ

Gene Symbol

PTPRJ

Additional DEP-1/CD148 Products

Product Documents for DEP-1/CD148 Antibody (A3)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for DEP-1/CD148 Antibody (A3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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