Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Predicted:

Primate (100%). Backed by our 100% Guarantee.

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all DDX24 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 325 and 375 of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 24 using the numbering given in entry NP_065147.1 (GeneID 57062).

Reactivity Notes

Orangutan (100%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DDX24 Antibody - BSA Free

Western Blot: DDX24 Antibody [NB100-2225]

Western Blot: DDX24 Antibody [NB100-2225]

Western Blot: DDX24 Antibody [NB100-2225] - Detection of Human DDX24 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T and NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DDX24 antibody NB100-2225 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: DDX24 Antibody [NB100-2225]

Immunoprecipitation: DDX24 Antibody [NB100-2225]

Immunoprecipitation: DDX24 Antibody [NB100-2225] - Detection of human DDX24 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DDX24 antibody NB100-2225 (lot 2) used for IP at 6 ug per reaction. DDX24 was also immunoprecipitated by a previous lot of this antibody (lot 1). For blotting immunoprecipitated DDX24, NB100-2225 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for DDX24 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: DDX24

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which shows little similarity to any of the other known human DEAD box proteins, but shows a high similarity to mouse Ddx24 at the amino acid level.

Alternate Names

ATP-dependent RNA helicase DDX24, DEAD (Asp-Glu-Ala-Asp) box polypeptide 24, DEAD box protein 24, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 24, EC 3.6.1, EC 3.6.4.13, S. cerevisiae CHL1-like helicase

Entrez Gene IDs

57062 (Human); 27225 (Mouse)

Gene Symbol

DDX24

UniProt

NP_065147.1 (Human)

Additional DDX24 Products

Product Documents for DDX24 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for DDX24 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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