Cytokeratin 5 Antibody (EP1601Y)

Novus Biologicals, part of Bio-Techne | Catalog # NB110-56916

Recombinant Monoclonal Antibody.
Discontinued Product
NB110-56916 has been discontinued. View all Cytokeratin 5 products.

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western

Cited:

Immunohistochemistry-Frozen, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # EP1601Y

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all Cytokeratin 5 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to residues on the C-terminus of human CK-5 was used as an immunogen.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cytokeratin 5 Antibody (EP1601Y)

Western Blot: Cytokeratin 5 Antibody (EP1601Y) [NB110-56916]

Western Blot: Cytokeratin 5 Antibody (EP1601Y) [NB110-56916]

Western Blot: Cytokeratin 5 Antibody (EP1601Y) [NB110-56916] - Expression of cytokeratins (CK) and GAPDH in HMGEC. Representative western blots show specific bands for GAPDH, CK1, Cytokeratin 5, CK6 and CK14. Image collected and cropped by CiteAb from the following publication (https://journals.plos.org/plosone/article?id=10.1371/journal.pone.01280…) licensed under a CC-BY license.
Immunocytochemistry/ Immunofluorescence: Cytokeratin 5 Antibody (EP1601Y) [NB110-56916]

Immunocytochemistry/ Immunofluorescence: Cytokeratin 5 Antibody (EP1601Y) [NB110-56916]

Immunocytochemistry/Immunofluorescence: Cytokeratin 5 Antibody (EP1601Y) [NB110-56916] - A431 cells.
Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (EP1601Y) [NB110-56916]

Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (EP1601Y) [NB110-56916]

Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (EP1601Y) [NB110-56916] - Cytokeratin 5 (EP1601Y) on prostate.
View 3 more images

Applications for Cytokeratin 5 Antibody (EP1601Y)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-250

Immunohistochemistry

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:200

Simple Western

1:100
Application Notes
Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 26419726) This is a high affinity, high titer antibody. Optimal dilutions should be determined by the end user. (Provided dilutions serve only as a suggestive starting point.).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.

Formulation, Preparation, and Storage

Purification

Tissue culture supernatant

Formulation

Culture supernatant in buffered aqueous solution

Preservative

No Preservative

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Cytokeratin 5

Keratins are a family of structurally related proteins that form the intermediate filament cytoskeleton in epithelial cells. The 58-kD keratin CK-5 is highly similar to other type II keratins and less similar to type I keratins and other intermediate filament proteins. The 58-kD keratin is regulated by retinoids in several tissues and is one of four keratins abundantly expressed in epidermal keratinocytes, where it may be important in maintaining structural integrity of the integument (1). Keratin 5 (CK-5) mRNA and protein are shown to be expressed in normal mammary epithelial cells in culture and are absent from tumor-derived cell lines. This makes CK-5 an important marker in the tumorigenic process, distinguishing normal from tumor cells, and decreased CK-5 expression correlates with tumorigenic progression (2). Dowling-Degos disease (DDD) is an autosomal dominant genodermatosis characterized by progressive and disfiguring reticulate hyperpigmentation of the flexures. Loss of function of CK-5 suggests a crucial role for keratins in the organization of cell adhesion, melanosome uptake, organelle transport, and nuclear anchorage (3).

Alternate Names

CK-5, DDD, EBS2, Keratin 5, KRT5

Entrez Gene IDs

3852 (Human); 110308 (Mouse); 369017 (Rat)

Gene Symbol

KRT5

UniProt

P13647

Additional Cytokeratin 5 Products

Product Documents for Cytokeratin 5 Antibody (EP1601Y)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Cytokeratin 5 Antibody (EP1601Y)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy Policy
Cookie Policy
Site Map
© Copyright 2026 Bio-Techne. All Rights Reserved. All trademarks and registered trademarks are the property of Bio-Techne and its brands unless otherwise specified.