Cytochrome P450 4A Antibody (10D10W7)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15847
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 10D10W7
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human Cytochrome P450 4A (CYP4A11) (Q02928). QFPCPPSHWLFGHIQELQQDQELQRIQKWVETFPSACPHWLWGGKVRVQLYDPDYMKVILGRSDPKSHGSYRFLAPWIGYGLLLLNGQTWFQHRRMLTPAF
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Cytochrome P450 4A Antibody (10D10W7)
![Western Blot: Cytochrome P450 4A Antibody (10D10W7) [NBP3-15847]](https://resources.bio-techne.com/images/products/Cytochrome-P450-4A-Antibody-10D10W7-Western-Blot-NBP3-15847-img0004.jpg "Western Blot: Cytochrome P450 4A Antibody (10D10W7) [NBP3-15847]")
Western Blot: Cytochrome P450 4A Antibody (10D10W7) [NBP3-15847]
Western Blot: Cytochrome P450 4A Antibody (10D10W7) [NBP3-15847] - Western blot analysis of extracts of various cell lines, using Cytochrome P450 4A (CYP4A11) Rabbit mAb (NBP3-15847) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry: Cytochrome P450 4A Antibody (10D10W7) [NBP3-15847] -
Immunohistochemistry: Cytochrome P450 4A Antibody (10D10W7) [NBP3-15847] - Immunohistochemistry analysis of Cytochrome P450 4A in paraffin-embedded mouse kidney tissue using Cytochrome P450 4A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry: Cytochrome P450 4A Antibody (10D10W7) [NBP3-15847] -
Immunohistochemistry: Cytochrome P450 4A Antibody (10D10W7) [NBP3-15847] - Immunohistochemistry analysis of Cytochrome P450 4A in paraffin-embedded rat kidney tissue using Cytochrome P450 4A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.Applications for Cytochrome P450 4A Antibody (10D10W7)
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Cytochrome P450 4A
Alternate Names
20-HETE synthase, CP4Y, CYP4A2, cytochrome P450 4A11, cytochrome P450, family 4, subfamily A, polypeptide 11, cytochrome P450, subfamily IVA, polypeptide 11, cytochrome P-450HK-omega, cytochrome P450HL-omega, fatty acid omega-hydroxylase, lauric acid omega-hydroxylase, P450HL-omega
Gene Symbol
CYP4A11
Additional Cytochrome P450 4A Products
Product Documents for Cytochrome P450 4A Antibody (10D10W7)
Product Specific Notices for Cytochrome P450 4A Antibody (10D10W7)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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