Cyclin A2 Antibody - BSA Free

Western Blot: Cyclin A2 Antibody [NBP1-31330]

Western Blot: Cyclin A2 Antibody [NBP1-31330] - 30 ug PC-12 whole cell lysate/extract 10% SDS-PAGE gel, antibody dilution 1:1000.

Western Blot: Cyclin A2 Antibody [NBP1-31330]

Western Blot: Cyclin A2 Antibody [NBP1-31330] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin A2 antibody diluted at a dilution of 1:1000.

Western Blot: Cyclin A2 Antibody [NBP1-31330]

Western Blot: Cyclin A2 Antibody [NBP1-31330] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin A2 antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: Cyclin A2 Antibody [NBP1-31330]

Western Blot: Cyclin A2 Antibody [NBP1-31330] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin A2 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: Cyclin A2 Antibody [NBP1-31330]

Immunohistochemistry-Paraffin: Cyclin A2 Antibody [NBP1-31330] - NCIN87 Xenograft, using cyclin A antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunoprecipitation: Cyclin A2 Antibody [NBP1-31330]

Immunoprecipitation: Cyclin A2 Antibody [NBP1-31330] - Sample: 1000 ug 293T whole cell lysate/extract A. 30 ug 293T whole cell lysate/extract, B. Control with 2. 5 ug of preimmune rabbit IgG, C. Immunoprecipitation of Cyclin A2 protein by 2. 5 ug of Cyclin A2 antibody 10% SDS-PAGE gel.

Western Blot: Cyclin A2 Antibody [NBP1-31330] -

Western Blot: Cyclin A2 Antibody [NBP1-31330] - Comparison of expression changes between young & old MRC-5 & HFF fibroblasts measured by RNA-seq, qRT-PCR, & Western Blots. (a) Four genes commonly downregulated & (b) 8 genes commonly upregulated in both cell lines. (a, b) The colors of the bars indicate the measurement technique (blue: RNA-seq; green: qRT-PCR; red: Western Blots/protein expression). Solid colored bars represent MRC-5 while shaded boxes represent HFF cells. The height of the bars corresponds to the logarithmic fold-change (FC) of expression between the first & the last PD investigated here (RNA-seq: log 2 RPKM FC; qRT-PCR: log 2− delta deltaCT; protein: log 2 expression ratio). Error bars indicate standard deviation from the mean. Changes statistically different comparing young & old PD (RNA-seq: DESeq; rRT-PCR/Protein: Student's t-test; n = 3) are indicated with an asterisk: ∗p < 0.05, ∗∗p < 0.01, & ∗∗∗p < 0.001. (c) The blots show the protein expression levels in MRC-5 & HFF cells at young compared to old PDs. The up- or downregulation was signified by the presence or absence of bands in Western Blots. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26339636), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Cyclin A2 Antibody [NBP1-31330] -

Cyclin A2 antibody detects Cyclin A2 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Cyclin A2 stained by Cyclin A2 antibody (NBP1-31330) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar= 10 um.