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CYC1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86872

Novus Biologicals, part of Bio-Techne
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NBP1-86872
NBP1-86872-25ul

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: NSEAARAANNGALPPDLSYIVRARHGGEDYVFSLLTGYCEPPTGVSLREGLYFNPYFPGQAIAMAPPIYTDVLEFDDGTPATMSQIAKDVCTFLRWASEPEHDHRKRMGLKMLMMMALLVPLVYTIKRHKWSVLKSRKLAYR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CYC1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: CYC1 Antibody [NBP1-86872]

Immunocytochemistry/ Immunofluorescence: CYC1 Antibody [NBP1-86872]

Immunocytochemistry/Immunofluorescence: CYC1 Antibody [NBP1-86872] - Staining of human cell line U-251 MG shows localization to mitochondria. Antibody staining is shown in green.
Immunohistochemistry: CYC1 Antibody [NBP1-86872]

Immunohistochemistry: CYC1 Antibody [NBP1-86872]

CYC1-Antibody-Immunohistochemistry-NBP1-86872-img0018.jpg
Immunohistochemistry-Paraffin: CYC1 Antibody [NBP1-86872]

Immunohistochemistry-Paraffin: CYC1 Antibody [NBP1-86872]

Immunohistochemistry-Paraffin: CYC1 Antibody [NBP1-86872] - Staining of human cerebral cortex shows moderate granular positivity in cytoplasm in neurons.

Applications for CYC1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Reviewed Applications

Read 1 review rated 4 using NBP1-86872 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CYC1

This is the heme-containing component of the cytochrome b-c1 complex, which accepts electrons from Rieskeprotein and transfers electrons to cytochrome c in the mitochondrial respiratory cha

Alternate Names

Complex III subunit 4, Complex III subunit IV, cytochrome c1, heme protein, mitochondrial, cytochrome c-1Cytochrome b-c1 complex subunit 4, Ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit, UQCR4

Gene Symbol

CYC1

Additional CYC1 Products

Product Documents for CYC1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CYC1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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