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CXCR3 Antibody

Catalog # NB100-56404 | Novus Biologicals a Bio-Techne Brand
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NB100-56404
NB100-56404SS

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Proximity Ligation Assay, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

Product Summary for CXCR3 Antibody

Immunogen

This antibody was developed against a synthetic peptide corresponding to amino acids 290-303 of human CXCR3.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CXCR3 Antibody

Western Blot: CXCR3 Antibody [NB100-56404] - HEK293T cells. Western blot image submitted by a verified customer review.
Immunocytochemistry/Immunofluorescence: CXCR3 Antibody [NB100-56404] - Brain microvascular endothelial cells isolated from WT and CXCR3 KO mice were stained with anti-CXCR3 (green) and anti-ZO-1 (blue) antibodies to analyze CXCR3 expression by immunofluorescence. Image from verified customer review.
Immunohistochemistry-Paraffin: CXCR3 Antibody [NB100-56404] - IHC analysis of a FFPE human spleen using 1:25 dilution of CXCR3 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of HIER using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (40X) was performed using Aperio AT2 (Leica Biosystems). Staining was performed by Histowiz.

Applications for CXCR3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

reported in scientific literature (PMID 31887215)

Immunohistochemistry-Frozen

reported in scientific literature (PMID 19234157)

Immunohistochemistry-Paraffin

1:10 - 1:500

Proximity Ligation Assay

reported in scientific literature (PMID 34433033)

Western Blot

1 - 3 ug/mL
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 5 using NB100-56404 in the following applications:

Published Applications

Read 10 publications using NB100-56404 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CXCR3

Chemokines play important roles in inflammation and critical for the recruitment of effector immune cells to sites of infection. Historically, CD183 was the third CXC chemokine receptor discovered and therefore, commonly designated as CXCR3. Binding of chemokines to CD183 induces cellular responses that are involved in leukocyte traffic, most notably integrin activation, cytoskeletal changes and chemotactic migration. The CXC chemokine receptor (CXCR) 3, a G-protein-coupled 7-transmembrane receptor, is expressed at high levels on activated and memory T cells, B cells, natural killer cells, and plasmacytoid monocytes.

Alternate Names

CD183, CXCR3, GPR9

Entrez Gene IDs

2833 (Human); 12766 (Mouse)

Gene Symbol

CXCR3

UniProt

Product Documents for CXCR3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CXCR3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for CXCR3 Antibody

Showing  1 - 2 of 2 FAQs Showing All
  • Q: Regarding recommended dilutions the manual contains: "Immunohistochemistry-Paraffin, Western Blot 1-3 ug/ml" Does it mean 1-3 ug/ml is recommended for both applications or there is simply no recommended dilution IHC-P?

    A: For our product NB100-56404, we did not have a dilution recommendation listed for IHC on our datasheet. I am sorry for any confusion. This application was added to our product based on a publication that actually uses this product at 1:200 in paraformaldehyde fixed frozen tissue samples: NCBI site.

  • Q: Which isoform of CXCR3 is this antibody specific for?

    A: NB100-56404 can only detect human CXCR3 isoforms 1 and 2 in WB and IHC-Fr, the validated applications.

Showing  1 - 2 of 2 FAQs Showing All
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