CXCR1/IL-8RA Antibody (SE2) - Chimeric - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12003

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # SE2

Format

Azide and BSA Free

Concentration

1 mg/ml

View all CXCR1/IL-8RA Antibodies »

Product Specifications

Immunogen

This antibody was raised by immunizing BALB/c mice with a BSA-conjugated peptide comprising of the N-terminal part of CXCR1/IL-8RA (amino acids 1-30).

Specificity

SE2 antibody is specific for CXCR1/IL-8RA and does not corss-react with IL-8RB. CXCR1/IL-8RA is a receptor to interleukin-8, which is a powerful neutrophils chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Description

Novus Biologicals Rabbit CXCR1/IL-8RA Antibody (SE2) - Chimeric - Azide and BSA Free (NBP3-12003) is a recombinant monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for CXCR1/IL-8RA Antibody (SE2) - Chimeric - Azide and BSA Free

Western Blot: CXCR1/IL-8RA Antibody (SE2)ChimericAzide and BSA Free [NBP3-12003]

Western Blot: CXCR1/IL-8RA Antibody (SE2)ChimericAzide and BSA Free [NBP3-12003]

Western Blot: CXCR1/IL-8RA Antibody (SE2) - Chimeric [NBP3-12003] - Human lymph node tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SE2 (NBP3-12003) at 0.1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: CXCR1/IL-8RA Antibody (SE2) - Chimeric - Azide and BSA Free [NBP3-12003]

Immunocytochemistry/ Immunofluorescence: CXCR1/IL-8RA Antibody (SE2) - Chimeric - Azide and BSA Free [NBP3-12003]

Immunocytochemistry/Immunofluorescence: CXCR1/IL-8RA Antibody (SE2) - Chimeric [NBP3-12003] - Immunofluorescence analysis of paraformaldehyde fixed human peripheral blood leukocytes on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of SE2 (NBP3-12003) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12003, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.
CXCR1/IL-8RA Antibody (SE2) - Chimeric - Azide and BSA Free

Flow Cytometry: CXCR1/IL-8RA Antibody (SE2) - Chimeric - Azide and BSA Free [NBP3-12003] -

Flow Cytometry: CXCR1/IL-8RA Antibody (SE2) - Chimeric - Azide and BSA Free [NBP3-12003] - Paraformaldehyde fixed human peripheral blood monocytes were stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-12003 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Applications for CXCR1/IL-8RA Antibody (SE2) - Chimeric - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: CXCR1/IL-8RA

CXCR1, also known as IL-8RA and CD181, is an approximately 60 kDa 7TM glycoprotein that functions as a receptor for the chemokine CXCL8/IL-8. It is expressed on neutrophils, monocytes, CD8 T cells, FoxP3+ CD4 Treg cells, mast cells, neuronal and glial cells, vascular endothelial cells, and melanoma. CXCR1 forms homodimers and heterodimers with CXCR2/IL-8RB. It can be cleaved from neutrophils in the lungs of cystic fibrosis patients to release fragments that promote CXCL8 production from airway epithelial cells. CXCR1 mediates neutrophil activation and chemotaxis to sites of inflammation as well as angiogenesis and melanoma invasiveness.

Long Name

Interleukin 8 Receptor A

Alternate Names

CD181, IL-8 RA, IL8RA

Gene Symbol

CXCR1

Additional CXCR1/IL-8RA Products

Product Documents for CXCR1/IL-8RA Antibody (SE2) - Chimeric - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CXCR1/IL-8RA Antibody (SE2) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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