CXCL10/IP-10/CRG-2 Antibody (JA10-82)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-67004
Recombinant Monoclonal Antibody.
![Immunocytochemistry/ Immunofluorescence: CXCL10/IP-10/CRG-2 Antibody (JA10-82) [NBP2-67004]](https://resources.bio-techne.com/images/products/CXCL10-IP-10-CRG-2-Antibody-JA10-82-Immunocytochemistry-Immunofluorescence-NBP2-67004-img0003.jpg "Immunocytochemistry/ Immunofluorescence: CXCL10/IP-10/CRG-2 Antibody (JA10-82) [NBP2-67004]")
![Immunohistochemistry-Paraffin: CXCL10/IP-10/CRG-2 Antibody (JA10-82) [NBP2-67004]](https://resources.bio-techne.com/images/products/CXCL10-IP-10-CRG-2-Antibody-JA10-82-Immunohistochemistry-Paraffin-NBP2-67004-img0002.jpg "Immunohistochemistry-Paraffin: CXCL10/IP-10/CRG-2 Antibody (JA10-82) [NBP2-67004]")
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Cited:
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JA10-82 expressed in HEK293
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide within Human CXCL10/IP-10/CRG-2 aa 49-98 / 98. (SwissProt: P02778 Human)
Localization
Secreted.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit CXCL10/IP-10/CRG-2 Antibody (JA10-82) (NBP2-67004) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for CXCL10/IP-10/CRG-2 Antibody (JA10-82)
![Immunocytochemistry/ Immunofluorescence: CXCL10/IP-10/CRG-2 Antibody (JA10-82) [NBP2-67004]](https://resources.bio-techne.com/images/products/CXCL10-IP-10-CRG-2-Antibody-JA10-82-Immunocytochemistry-Immunofluorescence-NBP2-67004-img0004.jpg "Immunocytochemistry/ Immunofluorescence: CXCL10/IP-10/CRG-2 Antibody (JA10-82) [NBP2-67004]")
Immunocytochemistry/ Immunofluorescence: CXCL10/IP-10/CRG-2 Antibody (JA10-82) [NBP2-67004]
Immunocytochemistry/Immunofluorescence: CXCL10/IP-10/CRG-2 Antibody (JA10-82) [NBP2-67004] - Staining IP10 in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.![Immunohistochemistry-Paraffin: CXCL10/IP-10/CRG-2 Antibody (JA10-82) [NBP2-67004]](https://resources.bio-techne.com/images/products/CXCL10-IP-10-CRG-2-Antibody-JA10-82-Immunohistochemistry-Paraffin-NBP2-67004-img0005.jpg "Immunohistochemistry-Paraffin: CXCL10/IP-10/CRG-2 Antibody (JA10-82) [NBP2-67004]")
Immunohistochemistry-Paraffin: CXCL10/IP-10/CRG-2 Antibody (JA10-82) [NBP2-67004]
Immunohistochemistry-Paraffin: CXCL10/IP-10/CRG-2 Antibody (JA10-82) [NBP2-67004] - Human tonsil tissue using anti-IP10 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.![Immunocytochemistry/ Immunofluorescence: CXCL10/IP-10/CRG-2 Antibody (JA10-82) [NBP2-67004]](https://resources.bio-techne.com/images/products/CXCL10-IP-10-CRG-2-Antibody-JA10-82-Immunocytochemistry-Immunofluorescence-NBP2-67004-img0001.jpg "Immunocytochemistry/ Immunofluorescence: CXCL10/IP-10/CRG-2 Antibody (JA10-82) [NBP2-67004]")
Immunocytochemistry/ Immunofluorescence: CXCL10/IP-10/CRG-2 Antibody (JA10-82) [NBP2-67004]
Immunocytochemistry/Immunofluorescence: CXCL10/IP-10/CRG-2 Antibody (JA10-82) [NBP2-67004] - Staining IP10 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Applications for CXCL10/IP-10/CRG-2 Antibody (JA10-82)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50-1:100
Immunohistochemistry-Paraffin
1:50-1:200
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CXCL10/IP-10/CRG-2
CXCL10/IP-10 is an ELR- angiostatic chemokine that interacts with CXCR3 to attract Th1 cells, eosinophils, monocytes, and NK cells to sites of inflammation. It is upregulated by activated T lymphocytes, neutrophils, splenocytes, keratinocytes, osteoblasts, astrocytes, endothelial cells, smooth muscle cells, and pancreatic beta cells. CXCL10 contributes to disease pathology in many chronic inflammatory disorders including rheumatoid arthritis, psoriasis, cancer, multiple sclerosis, diabetes, and cardiovascular disease.
Alternate Names
CRG-2, CRG2, IP-10
Gene Symbol
CXCL10
Additional CXCL10/IP-10/CRG-2 Products
Product Documents for CXCL10/IP-10/CRG-2 Antibody (JA10-82)
Product Specific Notices for CXCL10/IP-10/CRG-2 Antibody (JA10-82)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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