Citrulline Antibody (6C2.1) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59365
![Western Blot: Citrulline Antibody (6C2.1) [NBP2-59365]](https://resources.bio-techne.com/images/products/Citrulline-Antibody-6C2-1-Western-Blot-NBP2-59365-img0007.jpg "Western Blot: Citrulline Antibody (6C2.1) [NBP2-59365]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Non-species specific
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 6C2.1
Format
BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Synthetic L-Citrulline conjugated to Keyhole Limpet Hemocyanin (KLH).
Specificity
Specific for citrulline residues on modified proteins. Tested using citrulline conjugated Bovine Serum Albumin (BSA).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
Novus Biologicals Mouse Citrulline Antibody (6C2.1) - BSA Free (NBP2-59365) is a monoclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Citrulline Antibody (6C2.1) - BSA Free
Western Blot: Citrulline Antibody (6C2.1) [NBP2-59365]
Western Blot: Citrulline Antibody (6C2.1) [NBP2-59365] - Western Blot analysis of Citrulline-BSA Conjugate showing detection of 67 kDa Citrulline protein using Mouse Anti-Citrulline Monoclonal Antibody, Clone 6C2.1 (NBP2-59365). Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA (0.5 ug). Lane 3: BSA (2.0 ug). Lane 4: Citrulline-BSA (0.5 ug). Lane 5: Citrulline-BSA (2.0 ug). Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Citrulline Monoclonal Antibody (NBP2-59365) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.![Immunocytochemistry/ Immunofluorescence: Citrulline Antibody (6C2.1) [NBP2-59365]](https://resources.bio-techne.com/images/products/Citrulline-Antibody-6C2-1-Immunocytochemistry-Immunofluorescence-NBP2-59365-img0004.jpg "Immunocytochemistry/ Immunofluorescence: Citrulline Antibody (6C2.1) [NBP2-59365]")
Immunocytochemistry/ Immunofluorescence: Citrulline Antibody (6C2.1) [NBP2-59365]
Immunocytochemistry/Immunofluorescence: Citrulline Antibody (6C2.1) [NBP2-59365] - Analysis using Mouse Anti-Citrulline Monoclonal Antibody, Clone 6C2.1. Tissue: Embryonic kidney cells (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Citrulline Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) Citrulline Antibody (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.![Immunohistochemistry-Paraffin: Citrulline Antibody (6C2.1) [NBP2-59365]](https://resources.bio-techne.com/images/products/Citrulline-Antibody-6C2-1-Immunohistochemistry-Paraffin-NBP2-59365-img0006.jpg "Immunohistochemistry-Paraffin: Citrulline Antibody (6C2.1) [NBP2-59365]")
Immunohistochemistry-Paraffin: Citrulline Antibody (6C2.1) [NBP2-59365]
Immunohistochemistry-Paraffin: Citrulline Antibody (6C2.1) [NBP2-59365] - Tissue: Colon tissue. Species: Mouse. Fixation: Formalin fixed, paraffin embedded. Primary Antibody: Mouse Anti-Citrulline Monoclonal Antibody at 1:25 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: Alexa Fluor 488. Counterstain: DAPI (blue) nuclear stain. Magnification: 63X. (A) DAPI (blue) nuclear stain. (B) Citrulline Antibody (C) Composite.Applications for Citrulline Antibody (6C2.1) - BSA Free
Application
Recommended Usage
ELISA
1:1000
Immunocytochemistry/ Immunofluorescence
1:50
Immunohistochemistry
1:25
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.4), 50% Glycerol
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: Citrulline
Alternate Names
ACPA, Citrulline, L-Citrulline
Additional Citrulline Products
Product Documents for Citrulline Antibody (6C2.1) - BSA Free
Product Specific Notices for Citrulline Antibody (6C2.1) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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