Cholera Toxin Antibody (3D11) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB200-443

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Bacteria

Applications

ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 3D11

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Cholera Toxin Antibodies »

Product Specifications

Immunogen

Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with purified beta-subunit of cholera toxin.

Epitope

B-subunit of cholera toxin

Localization

Secreted

Specificity

Specific for the beta sub-unit of cholera toxin. Reacts with cholera toxin and E. coli heat labile enterotoxin.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Cholera Toxin Antibody (3D11) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Cholera Toxin

The B subunit of cholera toxin (CtxB) binds to a GM1-ganglioside receptor, a ubiquitous glycolipid cell surface receptor. This binding is widely accepted to initiate toxin action by triggering uptake and delivery of the toxin A subunit into cells. The beta chain has no toxic activity by itself. The holotoxin consists of a pentameric ring of B subunits whose central pore is occupied by the A subunit. The A subunit contains two chains, A1 and A2, linked by a disulfide bridge. The A subunit (and Cholera toxin) activates the adenylate cyclase enzyme in cells of the intestinal mucosa leading to increased levels of intracellular cAMP.

Alternate Names

Cholera enterotoxin gamma chain, Cholera enterotoxin subunit A, Cholera enterotoxin subunit B, Choleragenoid, toxA, toxB

Additional Cholera Toxin Products

Product Documents for Cholera Toxin Antibody (3D11) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cholera Toxin Antibody (3D11) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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