CENPE Antibody (CA8) - Chimeric - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-43562
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Cynomolgus Monkey
Applications
ELISA, Flow Cytometry
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # CA8
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
The original mouse parental antibody 118G03 was generated by immunizing SJL mice with recombinant human CENPE/TNFRSF17-Fc chimera (R&D 193-Fc) by using the RIMMS method (Rapid immunization multiple sites). A chimeric version of this antibody was generated and it is called CA8. (Uniprot# Q02224)
Specificity
This antibody binds human and cynomolgus monkey CENPE and does not bind TACI or BAFF-R proteins. CENPE acts as a receptor for TNFSF13B/BLyS/BAFF and TNFSF13/APRIL. CENPE expression is highest on terminally differentiated B cells. CENPE is involved in mediating the survival of plasma cells for maintaining long-term humoral immunity. It also activates NF-kappa-B and JNK. The expression of CENPE has been linked to a number of cancers, autoimmune disorders, and infectious diseases.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
Scientific Data Images for CENPE Antibody (CA8) - Chimeric - BSA Free
Immunocytochemistry/ Immunofluorescence: CENPE Antibody (CA8) - Chimeric - BSA Free [NBP3-43562] -
Immunocytochemistry/ Immunofluorescence: CENPE Antibody (CA8) - Chimeric - BSA Free [NBP3-43562] - Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on coverslips stained with NBP3-43562 (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, NBP3-43562, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody.
Flow Cytometry: CENPE Antibody (CA8) - Chimeric - BSA Free [NBP3-43562] -
Flow Cytometry: CENPE Antibody (CA8) - Chimeric - BSA Free [NBP3-43562] - Daudi cells were stained with the anti-unknown specificity antibody (isotype control, black line) or NBP3-43562 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and the cells were analyzed using a FACSCanto flow-cytometer.Applications for CENPE Antibody (CA8) - Chimeric - BSA Free
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CENPE
Long Name
Centromere-associated protein E
Alternate Names
CENP-E, Kinesin-7
Gene Symbol
CENPE
Additional CENPE Products
Product Documents for CENPE Antibody (CA8) - Chimeric - BSA Free
Product Specific Notices for CENPE Antibody (CA8) - Chimeric - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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