CD59 Antibody (193-27) - Chimeric - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12078
Recombinant Monoclonal Antibody
![Immunocytochemistry/ Immunofluorescence: CD59 Antibody (193-27) - Chimeric - Azide and BSA Free [NBP3-12078]](https://resources.bio-techne.com/images/products/CD59-Antibody-193-27-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-12078-img0001.jpg "Immunocytochemistry/ Immunofluorescence: CD59 Antibody (193-27) - Chimeric - Azide and BSA Free [NBP3-12078]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # 193-27
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
This antibody was raised by immunizng mice with stimulated human leukocytes.
Specificity
This antibody recognizes human CD59 which is a glycosyl phosphatidyl-inositol (GPI)-anchored cell surface protein. It regulates complement-mediated cell lysis, and is involved in lymphocyte signal transduction.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Description
Novus Biologicals Rabbit CD59 Antibody (193-27) - Chimeric - Azide and BSA Free (NBP3-12078) is a recombinant monoclonal antibody validated for use in IHC, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for CD59 Antibody (193-27) - Chimeric - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence: CD59 Antibody (193-27) - Chimeric - Azide and BSA Free [NBP3-12078]
Immunocytochemistry/Immunofluorescence: CD59 Antibody (193-27) - Chimeric [NBP3-12078] - Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 193-27 (NBP3-12078) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12078, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.
Flow Cytometry: CD59 Antibody (193-27) - Chimeric - Azide and BSA Free [NBP3-12078] -
Flow Cytometry: CD59 Antibody (193-27) - Chimeric - Azide and BSA Free [NBP3-12078] - Jurkat cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-12078 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.Applications for CD59 Antibody (193-27) - Chimeric - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgM format, for improved compatibility with existing reagents, assays and techniques.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Background: CD59
Alternate Names
CD59, HRF20, MACIF, MIC11, Protectin
Gene Symbol
CD59
Additional CD59 Products
Product Documents for CD59 Antibody (193-27) - Chimeric - Azide and BSA Free
Product Specific Notices for CD59 Antibody (193-27) - Chimeric - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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