CD300LG/Nepmucin Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-00118
![Immunoprecipitation: CD300LG/Nepmucin Antibody [NBP3-00118] - CD300LG/Nepmucin Antibody - Azide and BSA Free](https://resources.bio-techne.com/images/products/nbp3-00118_rabbit-polyclonal-cd300lg-nepmucin-antibody-23520251664788.png)
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Produced in rabbits immunized with purified, recombinant Human CD300LG/Nepmucin (Accession#: AAH25395.1; Met1-Arg247)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for CD300LG/Nepmucin Antibody - Azide and BSA Free
Immunoprecipitation: CD300LG/Nepmucin Antibody [NBP3-00118] -
Immunoprecipitation: CD300LG/Nepmucin Antibody [NBP3-00118] - CD300LG was immunoprecipitated using: Lane A:0.5 mg Jurkat Whole Cell Lysate 0.5 uL NBP3-00118 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP3-00118, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 38 kDa Observed band size: 38 kDaWestern Blot: CD300LG/Nepmucin Antibody [NBP3-00118] -
Western Blot: CD300LG/Nepmucin Antibody [NBP3-00118] - NBP3-00118 at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:36 kDa Observed band size:45 kDaApplications for CD300LG/Nepmucin Antibody - Azide and BSA Free
Application
Recommended Usage
Immunoprecipitation
1-2 uL/mg of lysate
Western Blot
1:500-1:1000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
0.2 um filtered solution in PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CD300g/Nepmucin
Alternate Names
CD300LG, CLM9, Nepmucin, TREM-4
Gene Symbol
CD300LG
Additional CD300g/Nepmucin Products
Product Documents for CD300LG/Nepmucin Antibody - Azide and BSA Free
Product Specific Notices for CD300LG/Nepmucin Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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