CARS Antibody (CL2304) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44481

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CL2304

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all CARS Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of A8MVQ3, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for CARS Antibody (CL2304) - Azide and BSA Free

CARS Antibody (CL2304) - Azide and BSA Free Immunohistochemistry-Paraffin: CARS Antibody [NBP3-44481]

Immunohistochemistry-Paraffin: CARS Antibody [NBP3-44481]

Analysis in human cerebral cortex and skeletal muscle tissues using NBP3-44481 antibody. Corresponding CARS RNA-seq data are presented for the same tissues.
CARS Antibody (CL2304) - Azide and BSA Free Western Blot: CARS Antibody [NBP3-44481]

Western Blot: CARS Antibody [NBP3-44481]

Lane 1: Marker [kDa]
Lane 2: Human cell line HeLa cytoplasmic fraction
Lane 3: Human cell line HeLa membrane fraction
Lane 4: Human cell line HeLa nuclear fraction
Lane 5: Human cell line HeLa chromatin fraction
Lane 6: Human cell line HeLa cytoskeletal fraction
CARS Antibody (CL2304) - Azide and BSA Free Western Blot: CARS Antibody [NBP3-44481]

Western Blot: CARS Antibody [NBP3-44481]

Lane 1: Marker [kDa]
Lane 2: Human cell line U-251 MG
View 4 more images

Applications for CARS Antibody (CL2304) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:5000 - 1:10000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CARS

CARS encodes a class 1 aminoacyl-tRNA synthetase, cysteinyl-tRNA synthetase. Each of the twenty aminoacyl-tRNA synthetases catalyzes the aminoacylation of a specific tRNA or tRNA isoaccepting family with the cognate amino acid. This gene is one of several located near the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq]

Alternate Names

CARS1, CysRS, cysteine translase, cysteine tRNA ligase 1, cytoplasmic, Cysteine--tRNA ligase, cysteinyl-tRNA synthetase, cysteinyl-tRNA synthetase, cytoplasmic, EC 6.1.1, EC 6.1.1.16, MGC:11246

Gene Symbol

CARS1

Additional CARS Products

Product Documents for CARS Antibody (CL2304) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CARS Antibody (CL2304) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.