Caldesmon/CALD1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85702

Novus Biologicals, part of Bio-Techne
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NBP1-85702
NBP1-85702-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Caldesmon/CALD1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SLKIEERAEFLNKSVQKSSGVKSTHQAAIVSKIDSRLEQYTSAIEGTKSAKPTKPAASDLPVPAEGVRNIKSMWEKGNVFSSPTAAGTPNKETAGLKVGVSSRINEWLTKTPDGNKSPAPKPSDLRPGDVSSKRNLWEKQSVDKVTS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit Caldesmon/CALD1 Antibody - BSA Free (NBP1-85702) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Caldesmon/CALD1 Antibody: Cited in 4 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Caldesmon/CALD1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Caldesmon/CALD1 Antibody [NBP1-85702]

Immunocytochemistry/ Immunofluorescence: Caldesmon/CALD1 Antibody [NBP1-85702]

Immunocytochemistry/Immunofluorescence: Caldesmon/CALD1 Antibody [NBP1-85702] - Staining of human cell line U-2 OS shows localization to plasma membrane and actin filaments. Antibody staining is shown in green.
Caldesmon/CALD1 Antibody - BSA Free Immunohistochemistry-Paraffin: Caldesmon/CALD1 Antibody - BSA Free [NBP1-85702]

Immunohistochemistry-Paraffin: Caldesmon/CALD1 Antibody - BSA Free [NBP1-85702]

Staining of human testis shows strong cytoplasmic positivity in peritubular myoid cells.
Caldesmon/CALD1 Antibody - BSA Free Immunohistochemistry-Paraffin: Caldesmon/CALD1 Antibody - BSA Free [NBP1-85702]

Immunohistochemistry-Paraffin: Caldesmon/CALD1 Antibody - BSA Free [NBP1-85702]

Staining of human duodenum shows strong cytoplasmic positivity in smooth muscle cells.
View 7 more images

Applications for Caldesmon/CALD1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Caldesmon/CALD1

Caldesmon is a smooth muscle and nonmuscle regulatory protein that interacts with actin, myosin, tropomyosin, and calmodulin (1,2). Smooth muscle caldesmon is an elongated molecule with a calmodulin, tropomyosin, and actin-binding region at the C-terminus and a myosin-binding domain at the N-terminus (3). Caldesmon stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, caldesmon inhibits the actomyosin ATPase by binding to F-actin. This inhibition is reduced by calcium-calmodulin and is promoted by tropomyosin (4).

Alternate Names

CALD1, CDM, HCAD, LCAD, NAG22

Gene Symbol

CALD1

Additional Caldesmon/CALD1 Products

Product Documents for Caldesmon/CALD1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Caldesmon/CALD1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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